Biotinylated Anti-Human IL-8 (CXCL8) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P28BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-8. Anti-Human IL-8 specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human IL-8 (CXCL8) (72 a.a.) (PeproTech catalog# 200-08M) Sandwich ELISA: To detect Human IL-8 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IL-8 (500-P28) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-8. Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Human, References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Angiogenesis/Cardiovascular Apoptosis Chemotaxis Wound Healing Allergy Transplantation product.subtitle.recentcitations First Author McNaughton, E F Title Novel Anti-Inflammatory Peptides Based on Chemokine-Glycosaminoglycan Interactions Reduce Leukocyte Migration and Disease Severity in a Model of Rheumatoid Arthritis. Citation Journal of immunology (Baltimore, Md. : 1950); 200(9) pg3201-3217 PubMed Id 29572348 First Author Vanheule, V Title The Positively Charged COOH-terminal Glycosaminoglycan-binding CXCL9(74-103) Peptide Inhibits CXCL8-induced Neutrophil Extravasation and Monosodium Urate Crystal-induced Gout in Mice. Citation The Journal of Biological Chemistry; 290(35) pg21292-304 PubMed Id 26183778 First Author Dyer, D P Title TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8. Citation Journal of immunology (Baltimore, Md. : 1950); 192(5) pg2177-85 PubMed Id 24501198
