Biotinylated Anti-Human SDF-1α (CXCL12) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P87ABT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human SDF-1α. Anti-Human SDF-1α specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli-derived, 8.0 kDa Recombinant Human SDF-1α (CXCL12) (PeproTech catalog# 300-28A) Sandwich ELISA: To detect Human SDF-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human SDF-1α (500-P87A) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human SDF-1α. Western Blot: To detect Human SDF-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Human, References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest AIDS/HIV Chemotaxis Immune System Inflammation Neurobiology Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Wang, Z Title Carcinomas assemble a filamentous CXCL12-keratin-19 coating that suppresses T cell-mediated immune attack. Citation Proceedings of the National Academy of Sciences of the United States of America; 119(4) PubMed Id 35046049 First Author Sánchez-Martín, L Title The chemokine CXCL12 regulates monocyte-macrophage differentiation and RUNX3 expression. Citation Blood; 117(1) pg88-97 PubMed Id 20930067