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Biotinylated Polyclonal Antibodies

Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit)

Product Details

Catalog Number: 500-P133BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsRANKL. Anti-Human sRANKL specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 20.0 kDa Recombinant Human sRANK Ligand (PeproTech catalog# 310-01)

Sandwich ELISA: To detect hsRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human sRANKL (500-P133) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsRANKL.

Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit) Sandwich ELISA

Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit) Western Blot Unreduced Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit) Western Blot Reduced

Note:

1mg will be provided as 2x500μg.

Cross Reactivity Cited in References: Research Interest

Country Of Origin: USA

Not for human use.