Biotinylated Anti-Human sTNF Receptor Type I 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P143BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-Receptor I. Anti-Human sTNF-Receptor I specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli-derived, 18.3 kDa Recombinant Human TNF Receptor Type I (PeproTech catalog# 310-07) Sandwich ELISA: To detect hsTNF-Receptor I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human sTNF-Receptor I (500-P143) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsTNF-Receptor I. Western Blot: To detect hsTNF-Receptor I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-Receptor I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Inflammation Neurobiology Receptors TNF Superfamily Recent Citations First Author Lobito, A A Title Abnormal disulfide-linked oligomerization results in ER retention and altered signaling by TNFR1 mutants in TNFR1-associated periodic fever syndrome (TRAPS). Citation Blood; 108(4) pg1320-7 PubMed ID 16684962