Produced from sera of rabbits immunized with highly pure recombinant Human Visfatin. Anti-Human Visfatin specific antibody was purified by affinity chromatography and then biotinylated.
E.coli-derived, 52.6 kDa Recombinant Human Visfatin (PeproTech catalog# 130-09)
To detect Human Visfatin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human Visfatin (500-P222) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Visfatin.
To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.