Cell Culture Media: Research, Products, & Supplements


PeproTech Cell Culture Media Products

Redefining Media Quality Utilizing PeproTech Cytokine Products

 

PeproGrow Media Bottles As a leading researcher and manufacturer of cytokine products, PeproTech is pleased to offer media that contain our recombinant proteins and/or growth factors. These media formulations have been engineered by PeproTech's scientists and collaborators to provide the basic nutrients to grow and support several specific cell types.

 

PeproGrow™ hMSC Medium

Maintenance Medium for Human Mesenchymal Stem Cells

Human MSC Basal Media and Growth Factor vial

•  Complete Media

•  Superior growth rates

•  Xeno-free, phenol red-free

•  Phenotypic integrity

•  Maintained multipotency

•  Affordable and competitive pricing

•  Works well with adipose tissue-derived, bone marrow-derived, umbilical cord-derived, placental-derived,
    and urine-derived MSCs

•  Developed in collaboration with American CryoStem Corporation

                                

PeproGrow hMSC (Mesenchymal Stem Cell) Medium is a xeno-free, human serum-containing, phenol red-free complete media formulation originally designed for the in vitro expansion of adipose-derived human mesenchymal stem cells (ADMSCs) in the multipotent state. This media formulation has been shown to be suitable for the sustained growth of adipose tissue-derived, bone marrow-derived, umbilical cord-derived, placental-derived, and urine-derived MSCs in both adherent and suspension culture. For optimal results, culturing should be conducted on a surface coated with PeproTech's Animal-Free Human Vitronectin Matrix as a surface-coating reagent; however, other suitable extracellular matrix (ECM) proteins, such as fibronectin or vitronectin, can be used. PeproGrow hMSC Medium was designed and developed by PeproTech in collaboration with American CryoStem Corporation, and is supplied as a 500mL bottle of PeproGrow hMSC Basal Medium (Catalog# BM-XF-HMSC-500) containing a human serum component, and a separate, lyophilized vial of animal-free PeproGrow hMSC Growth Factor Supplement (Catalog# GF-XF-HMSC-500). The addition of the separate, lyophilized growth factor supplement to the basal medium results in a complete medium containing all growth factors and supplements necessary for optimal expansion of human mesenchymal stem cells in culture.

 

PeproGrow™ hMSC Medium 500mL Kit

Catalog #XF-hMSC-500

 
     Basal Medium BM-XF-hMSC-500 500mL
     Growth Factor Component GF-XF-hMSC-500 Vial for 500mL Basal Medium

 


PeproGrow™ hMSC Medium Figures and Descriptions


Figure 1 

Fluorescent immunostaining of adipose tissue-derived mesenchymal stem cells (ADMSCs) grown in PeproGrow hMSC Medium.

Fluorescent Immunostaining of Adipose Derived Mesenchymal Stem Cells in PeproGrow hMSC Medium


Cells were grown for 4 days on Animal-Free Human Vitronectin Matrix-coated chamber slides at 37ºC in an atmosphere of 5% O2 and 5% CO2.  Following growth, cells were rinsed once with PBS to remove debris and dead cells, then fixed for 20 minutes at RT with freshly-prepared 4% paraformaldehyde in PBS/HEPES, pH 7.4.  Following fixation, cells were rinsed 3 times with PBS, blocked/permeabilized with 1x PERM/WASH (BD), and subjected to primary antibody staining (BioGems) at recommended concentrations overnight at 4ºC. The following day, samples were washed 3 times with PBS, and incubated with secondary antibodies (ThermoFisher/Molecular Probes) diluted in 1:4 PERM/Wash:PBS as follows:  all goat anti-rabbit antibodies were used at 1:1000, whereas goat anti-mouse isotypes were used at 1:500 dilutions. The samples were then washed 1 time with PBS containing 500 ng/ml DAPI, twice more in PBS, alone, and once in DI water to remove salts prior to mounting/cover-slipping in Aqua-Poly Mount (Polysciences) with 12 mm glass coverslips. The slides were allowed to dry overnight at RT prior to imaging using an Olympus IX71 Inverted microscope equipped with an inverted cooled CCD camera.  Images were captured using iVision software (12 bit), pseudo-colored (DAPI = blue, AF488 = green, AF594 = red), and then exported to 8 bit format.  In order to ensure specificity, all image capture times were adjusted to, or below times, that of the isotype controls that revealed minimal background staining levels.  As shown, ADMSCs grown in PeproGrow hMSC Medium were strongly positive for CD13, CD29, CD44, CD73, CD90, CD105, and CD146.


Figure 2

Fluorescent immunostaining of bone marrow-derived mesenchymal stem cells (BDMSCs) grown in PeproGrow hMSC Medium.

Fluorescent immunostaining of Bone Marrow Derived Mesenchymal Stem Cells in PeproGrow hMSC Medium


Cells were grown for 4 days on Animal-Free Human Vitronectin Matrix-coated chamber slides at 37ºC in an atmosphere of 5% O2 and 5% CO2.  Following growth,  the cells were rinsed once with PBS to remove debris and dead cells, then fixed for 20 minutes at RT with freshly-prepared 4% paraformaldehyde in PBS/HEPES, pH 7.4.  Following fixation, cells were rinsed 3 times with PBS, blocked/permeabilized with 1x PERM/WASH (BD), and subjected to primary antibody staining (BioGems) at recommended concentrations overnight at 4ºC. The following day, samples were washed 3 times with PBS, and incubated with secondary antibodies (ThermoFisher/Molecula rProbes) diluted in 1:4 PERM/ Wash:PBS as follows:  all goat anti-rabbit antibodies were used at 1:1000, whereas goat anti-mouse isotypes were used at 1:500 dilutions. The samples were then washed 1 time with PBS containing 500 ng/ml DAPI, twice more in PBS, alone, and once in DI water to remove salts prior to mounting/cover-slipping in Aqua-Poly Mount (Polysciences) with 12 mm glass coverslips. The slides were allowed to dry overnight at RT prior to imaging using an Olympus IX71 Inverted microscope equipped with an inverted cooled CCD camera.  Images were captured using iVision software (12 bit), pseudo-colored (DAPI = blue, AF488 = green, AF594 = red), and then exported to 8 bit format.  In order to ensure specificity, all image capture times were adjusted to, or below times, that of the isotype controls that revealed minimal background staining levels.  As shown, BDMSCs grown in PeproGrow hMSC Medium were strongly positive for CD13, CD29, CD44, CD73, CD90, CD105, and CD146.


Figure 3

Fluorescent immunostaining of placental-derived mesenchymal stem cells (PDMSCs) grown in PeproGrow hMSC Medium.

Fluorescent Immunostaining of Placental Derived Mesenchymal Stem Cells in PeproGrow hMSC Medium


Cells were grown for 4 days on Animal-Free Human Vitronectin Matrix-coated chamber slides at 37ºC in an atmosphere of 5% O2 and 5% CO2. Following growth,  the cells were rinsed once with PBS to remove debris and dead cells, then fixed for 20 minutes at RT with freshly-prepared 4% paraformaldehyde in PBS/HEPES, pH 7.4. Following fixation, cells were rinsed 3 times with PBS, blocked/permeabilized with 1x PERM/WASH (BD), and subjected to primary antibody staining (BioGems) at recommended concentrations overnight at 4ºC. The following day, samples were washed 3 times with PBS, and incubated with secondary antibodies (ThermoFisher/Molecular Probes) diluted in 1:4 PERM/Wash:PBS as follows: all goat anti-rabbit antibodies were used at 1:1000, whereas goat anti-mouse isotypes were used at 1:500 dilutions. The samples were then washed 1 time with PBS containing 500 ng/ml DAPI, twice more in PBS, alone, and once in DI water to remove salts prior to mounting/cover-slipping in Aqua-Poly Mount (Polysciences) with 12 mm glass coverslips. The slides were allowed to dry overnight at RT prior to imaging using an Olympus IX71 Inverted microscope equipped with an inverted cooled CCD camera.  Images were captured using iVision software (12 bit), pseudo-colored (DAPI = blue, AF488 = green, AF594 = red), and then exported to 8 bit format.  In order to ensure specificity, all image capture times were adjusted to or below times that of the isotype controls that revealed minimal background staining levels.  As shown, PDMSCs grown in PeproGrow hMSC Medium were strongly positive for CD13, CD29, CD44, CD73, CD90, CD105, and CD146.

 


PeproGrow™ Animal-Free Low Protein hESC Medium 

Maintenance Medium for hESCs and hiPSCs


PeproGrow Animal-Free Low Protein hESC Basal Media Bottle
•  Animal component-free, phenol red-free, insulin-free

•  Complete and chemically-defined

•  Animal-free basal media available as a low protein medium

•  High plating efficiency

•  High quality recombinant growth factors from the leading manufacturer

•  Developed in collaboration with and used in the Rutgers Stem Cell Training Course

•  Based on the E8® formulation

PeproGrow Animal-Free Low Protein hESC Medium is an animal component-free, serum- and phenol red-free medium of a complete, chemically-defined formulation designed for feeder-free maintenance and expansion of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs).  This medium formulation is best used with PeproTechís Animal-Free Human Vitronectin Matrix as a surface-coating reagent; however, it can also be used with Corning Matrigel® or Synthemax® II-SC.  The medium is intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (Nanog+/Lin28+/Tra-1-60+ or SSEA4+/Oct4+), and demonstrates less than 15% spontaneous differentiation as indicated by immunofluorescent staining.  Similar to the PeproGrow hESC medium, this proprietary medium formulation includes relevant growth factors, such as FGF2 (FGF-basic), and do not contain the insulin found in the majority of other hESC/iPSC media currently available on the market. PeproGrow Animal-Free Low Protein hESC Medium was designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University.  This medium contains the minimal, essential components required for stem cell culture maintenance and is developed in the likeness of the E8® formulation developed by the laboratory of Dr. James Thomson at the University of Wisconsin, Madison.* This kit is supplied as a 100mL, or 500mL, bottle of basal medium, and a separate, lyophilized growth factor component. Additional companion products, such as the Animal-Free Human Vitronectin Matrix and Buffer Kit, and the Cell Passaging/Non-Enzymatic Detachment Buffer, are available separately.

* E8 is a registered trademark of the Wisconsin Alumni Research Foundation.

 

PeproGrow™ Animal-Free Low Protein hESC Medium Kit

Based on the E8® Formulation*
     Kit Catalog Number:   AF-LP-hESC-500  
  For 500mL size Low Protein Basal Medium AF-BM-LP-hESC-500 500mL
  Animal-Free Growth Factor Component AF-GF-hESC-500  Vial for 500mL Basal Medium
     Kit Catalog Number:   AF-LP-hESC-100  
     For 100mL size Low Protein Basal Medium AF-BM-LP-hESC-100 100mL
  Animal-Free Growth Factor Component AF-GF-hESC-100 Vial for 100mL Basal Medium

 


Companion Products for PeproGrow™ Animal-Free Low Protein hESC Media

Animal-Free Human Vitronectin Matrix and Buffer Kit

Catalog #AF-VMB-220

 
     Animal-Free Human Vitronectin Matrix   500ug
     PBS + Kolliphor P 188   220mL

Cell Passaging/Non-Enzymatic Detachment Buffer

Catalog #CPD-125

 
     Buffer contains PBS+HEPES+EDTA   125mL

 


PeproGrow™ Animal-Free Low Protein hESC Medium Figures and Descriptions

Flow Cytometry of HFF cells derived from lentiviral reprogramming of human foreskin fibroblasts,
and cultured in each medium and coating matrix as described. Similar flow cytometry results were also obtained with H1 hESCs. 


Figure 4 

PeproGrow Animal-Free Low Protein hESC Medium with PeproTech Animal-Free Human Vitronectin Matrix

PeproGrow Animal-Free Low Protein hESC Medium with PeproTech Vitronectin Matrix Flow Cytometry


Figure 5 

Competitor Medium with PeproTech Animal-Free Human Vitronectin Matrix

Competitor Medium with Animal-Free Human Vitronectin Matrix Flow Cytometry 


Figure 6 

PeproGrow hESC Media with Corning Matrigel

PeproGrow hESC Media with Corning Matrigel Flow Cytometry

 

Immunostaining of pluripotent stem cells cultured in each medium below using PeproTech Animal-Free Human Vitronectin Matrix-coated plates/dishes.


 Figure 7                                                               Figure 8 

Immunostaining of pluripotent stem cells using PeproTech Animal-Free Human Vitronectin Matrix

 

PeproGrow™ hESC Medium

Maintenance Medium for hESCs and hiPSCs

PeproGrow hESC Basal Medium Bottle

•  Phenol red-free and insulin-free

•  Complete and chemically-defined

•  High plating efficiency

•  High quality recombinant growth factors from the leading manufacturer

•  Developed in collaboration with and used in the Rutgers Stem Cell Training Course

                                

PeproGrow hESC is a serum- and phenol red-free medium of a complete, chemically-defined formulation designed for feeder-free maintenance and expansion of both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) using Corning Matrigel® as a surface-coating matrix. This medium is intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (SSEA4+/Oct4+), and demonstrates less than 15% spontaneous differentiation as indicated by flow cytometry. The proprietary formulation of the medium includes relevant growth factors, such as FGF2 (FGF-basic), but does not contain the insulin found in the majority of other hESC/iPSC media currently available on the market. PeproGrow hESC, which was designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University, is supplied as a 100mL, or 500mL, bottle of basal medium and a separate, lyophilized growth factor component. 


PeproGrow™ hESC Medium Figures and Descriptions


Figure 9 

The growth of H1 hESCs expressing turboGFP-NEO under the control of the Oct4 promoter. 

Growth of H1 hESCs

H1 hESCs modified by lentivirus to contain the Oct4 promoter driving turboGFP, were cultured in PeproGrow hESC medium, were passaged using dispase and plated in PeproGrow hESC medium containing 2 µM Y-27632 onto Corning Matrigel® coated 6 well dishes. Cultures were fed daily and photographed 24, 48, and 72 hours post split. Green fluorescence represents maintenance of pluripotency as indicated by Oct4 promoter activity. PeproGrow hESC maintains cells in the pluripotent state like the competitor media and allows the cells to plate out better (faster confluency).


Figure 10 

Cell Morphology of hESCs cultured in PeproGrow hESC medium. 

Cell Morphology of hESCs culture in PeproGrow hESC Medium

This medium contains a unique blend of cytokines that at first may alter the cellular or colony appearance, making them more  flat, and elastic, however this morphological change appears less dramatic as the cell density increases, and over several passages. Stem cells will form standard tight circular colonies (left), and have expected morphology with large nucleus and small cytoplasm (right).


Figure 11 

Immunostaining of iPSCs cultured in PeproGrow hESC medium. 

Immunostaining of iPSCs cultured in PeproGrow hESC Medium

Cells were plated on 24 well dishes, para-formaldehyde fixed and then stained with DAPI and other indicated antibodies, which indicate cell pluripotency.


Specifications

     Sterility Negative
     pH 7.35-7.40
     Osmolality 340-350 mOsm

 

 

 




PeproGrow™ hESC Medium Kit

PeproGrow™ hESC Medium 500mL Kit hESC-500   
                                   Basal Medium BM-hESC-500 500mL
                                   Growth Factor Component GF-hESC-500 Vial for 500mL Basal Medium
PeproGrow™ hESC Medium 100mL Kit hESC-100   
                                   Basal Medium BM-hESC-100 100mL
                                   Growth Factor Component GF-hESC-100 Vial for 100mL Basal Medium

 

 
 

PeproGrow™ Endothelial Medium

Maintenance Media for Endothelial Cells

PeproGrow™ EPC, PeproGrow™ MacroV, and PeproGrow™ MicroV

PeproGrow Endothelial Media Bottles

•  Complete media

•  Antibiotic-free, antimycotics-free, antifungal-free, and phenol red-free

•  Maintain outstanding endothelial cell morphology and function

•  Increased activity of endothelial nitric oxide synthase (eNOS)

                                

PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro cultivation of: endothelial progenitor cells (EPCs; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPCs; growing cells at rates that exceed commercially available media. 

PeproTech's endothelial cell culture media kit is supplied as a 500mL bottle of basal medium and a separate growth supplement bottle that contains various essential growth factors and components for endothelial cell growth. Adding the growth supplement to the basal medium results in the complete culture medium. PeproTech's endothelial media do not contain antibiotics, antimycotics, antifungals, or phenol red, as these components can cause cell stress and masking effects that may reduce complete medium shelf life and influence experimental results.

 


Media Products

PeproGrow™ EPC Kit (ENDO-BM & GS-EPC)

Catalog #700-EPC

 
     Basal Medium ENDO-BM  500mL
     Growth Supplement EPC GS-EPC*  75mL

PeproGrow™ MacroV Kit (ENDO-BM & GS-MacroV)

Catalog #700-MacroV

 
     Basal Medium ENDO-BM 500mL
     Growth Supplement MacroV GS-MacroV* 25mL

PeproGrow™ MicroV Kit (ENDO-BM & GS-MicroV)

Catalog #700-MicroV

 
     Basal Medium ENDO-BM 500mL
     Growth Supplement MicroV GS-MicroV* 35mL
  *Contains FBS


PeproGrow™ Endothelial Media Figures and Descriptions

 

Figure 12 

Endothelial Progenitor Cell, EPC Proliferation. 

Endothelial Progenitor Cell Proliferation Using PeproGrow EPC Kit

EPCs were seeded onto fibronectin-coated plates, and incubated for 7 days in the PeproGrow EPC Kit and a competitorís medium. Figure 10(A) represents the proliferative ability of EPCs assessed 7 days after cell cultivation using the XTT assay according to the manufacturer protocol. The proliferative ability of EPCs was expressed as the average optical density (O.D.) calculated using a plate reader for two independent assays run in triplicate.  Figure 12(B) represents a standard Western Blot assay.


Figure 13 

Endothelial Progenitor Cell, EPC Characterization.

Endothelial Progenitor Cell Characterization Using PeproGrow EPC Kit

EPCs were cultured for 7 days. Each description correlates to images from left to right: Panel 1: Acetylated LDL uptake by adherent spindle-shaped EPCs, FITC-conjugated lectin UEA-1 binding to the surface of EPCs, and positive double-stained (merged image) EPCs for acetylated LDL uptake and lectin binding. Panel 2: Immunofluorescence detection of CD34 antigen (red), KDR (green) on the surface of EPCs, and merged image. Panel 3: Immunofluorescence detection of eNOS on a single non-stained EPC (green). Panel 4: Immunofluorescence detection of CD34 antigen on the EPCs surface (red), eNOS (green), and merged image. The EPCs nuclei were stained with the blue fluorescent DNA dye DRAQ5™.  

 

Figure 14 

Macrovascular Endothelial Cell, HUVEC Proliferation.

Macrovascular Endothelial Cell HUVEC Proliferation Using PeproGrow MacroV Kit

HUVECs were seeded onto fibronectin-coated plates and incubated for 7 days in the PeproGrow MacroV Kit, and two competitorsí media. The proliferative ability of HUVECs was assessed 7 days after cell cultivation using the XTT assay following the manufacturerís protocol. The proliferative ability of HUVECs was expressed as the average of optical intensity (O.D.) calculated using a plate reader from two independent assays run in triplicate. 


Figure 15 

Microvascular Endothelial Cell, HDMEC Proliferation.

Microvascular Endothelial Cell HDMEC Proliferation Using PeproGrow MacroV Kit

HDMECs were seeded onto fibronectin-coated plates and incubated for 7 days in the PeproGrow MacroV Kit and a competitorís medium. Figure 15(A) represents the proliferative ability of HDMECs assessed 7 days after cell cultivation using the XTT assay following the manufacturerís protocol. The proliferative ability of HDMECs was expressed as the average of optical density (O.D.) calculated using a plate reader from two independent assays run in triplicate. Figure 15(B) represents a standard Western Blot assay.

 


Recommended Cell Types

PeproGrow EPC is recommended for Endothelial Progenitor Cells:

Human Endothelial Progenitor Cells (hEPCs)

 

PeproGrow MacroV is recommended for Macrovascular Endothelial Cells:

Human Umbilical Vein Endothelial Cells (HUVECs)

Human Umbilical Artery Endothelial Cells (HUAECs)

Human Aortic Endothelial Cells (HAoECs)

Human Pulmonary Artery Endothelial Cells (HPAECs)

Human Saphenous Vein Endothelial Cells (HSaVECs)

 

PeproGrow MicroV is recommended for Microvascular Endothelial Cells:

Human Coronary Artery Endothelial Cells (HCAECs)

Human Pancreatic Microvascular Endothelial Cells (HPaMECs)

Human Dermal Microvascular Endothelial Cells (HDMECs)

Human Pulmonary Microvascular Endothelial Cells (HPMECs)

Human Dermal Lymphatic Endothelial Cells (HDLECs)

Human Brain Microvascular Endothelial Cells (HBMECs) 


Specifications

     Sterility Absence of bacteria, mycoplasma and fungi
     pH 7.4-7.8
     Osmolality 260-280 mOsm
     Endotoxin Testing <0.5 EU/mL

 

 

 

 

 

 

PeproGrow™ HEK293 Medium

Maintenance Medium for HEK293 Cells

•  Animal component-free, serum-free, protein-free, chemically-defined medium

•  Complete medium containing L-alanyl-L-Glutamine for direct product use

•  High recombinant protein expression

                                

PeproGrow HEK293 is an animal component-free, protein-free, serum-free, chemically-defined, complete medium formulation for the in vitro cultivation of HEK293 cells (Thermo Fisher Scientific FreeStyle 293-F cells, catalog number R790-07). This medium is intended for recombinant protein expression in suspension culture, which is recommended for a 5 day batch culture with a seeding density of 0.6 x106 cells/mL. This ready-to-use medium contains  L-alanyl-L-Glutamine, amino acids, vitamins, and salts.  An adaptation process is not required for Thermo Fisher Scientific FreeStyle™ 293-F cells.


PeproGrow™ HEK293 Medium Figures and Descriptions


Figure 16 

Figures 16 (A, B, C) illustrate HEK293 BMP-6 expression in PeproGrow HEK293 medium and competitor CD-293 medium.

HEK293 BMP-6 expression in PeproGrow HEK293 medium



PeproGrow™ HEK293 Media

 

 
     PeproGrow™ HEK293 Catalog #AF-CD-HEK293 1L

 

PeproGrow™ CHO Medium

Maintenance Medium for CHO-S cell lines

 

PeproGrow AF-CHO Basal Media Bottle

•  Animal component-free, serum-free, and protein-free 

•  Complete medium containing L-alanyl-L-Glutamine for direct product use

•  High recombinant protein expression

                                

PeproTech offers PeproGrow AF-CHO, for the in vitro cultivation of Chinese Hamster Ovary-S cells (Thermo Fisher Scientific catalog numbers 11619-012, R800-07, or A11557-01). This medium is intended for recombinant protein expression in suspension culture. PeproGrow AF-CHO is an animal component-free, serum-free, protein-free, complete medium formulation. This ready-to-use medium contains L-alanyl-L-Glutamine, amino acids, vitamins, salts and non-animal derived hydrolysates, which are recommended for a 5 day batch culture with a seeding density of 0.5 x106 cells/mL. An adaptation process is not required for Thermo Fisher Scientific CHO-S cells.


PeproGrow™ CHO Medium Figures and Descriptions


Figure 17 

Figures 17 (A, B) illustrate CHO-S cell density and percent viability of C1 Inhibitor producing cells in PeproGrow AF-CHO and competitor CD-CHO.

CHO-S cell density and percent viability in PeproGrow AF-CHO media

Cell expression varies by protein product.


Figure 18 

The western blot illustrates CHO-S cell culture sample production of C1 Inhibitor post 5 days.

Western Blot of PeproGrow AF-CHO cell culture sample production

  Lane 1: PeproGrow AF-CHO

  Lane 2: Commercial CD-CHO

 

PeproGrow™ CHO Media

 

 
     PeproGrow™ AF-CHO Catalog #AF-CHO 1L



PeproGrow-1 Serum-Free Cell Culture Supplement Kit

Serum-free cell culture supplement kit for adherent HEK293, HeLa, and A549 cells

•  Serum-free, animal-free, and protein-free medium supplement

•  Chemically-defined medium supplement

                                


PeproGrow-1 (catalog number 700-C100) is a serum-free cell culture media supplement formulation designed to sustain the growth of adherent mammalian cell lines, and has been tested with HEK293, HeLa, and A549 cells.  This kit may potentially improve the culturing conditions of other adherent cells, however suitability for cells other than those pre-tested is not guaranteed. PeproGrow-1 is an animal component-free, protein-free, chemically-defined formulation.  This kit is intended to be used with DMEM/F12 basal media (Thermo Fisher Scientific catalog #10565, or catalog #31331 for customers located outside the USA) and contains enough material to supplement 10L of media.

Note: DMEM/F12 media can contain phenol red, or phenol red can be added at the customerís discretion. DMEM/F12 media should not contain HEPES. Another vendorís DMEM/F12 can be purchased, however, PeproTech only tests PeproGrow-1 (Catalog # 700-C100) using Thermo Fisher Scientific DMEM/F12. 

 


PeproGrow-1 Serum-Free Cell Culture Supplement Kit Components:

•  Serum Replacement Solution (Catalog #SR-100):

This 100x serum replacement solution contains non-animal derived, chemically-defined salts, designed to replace serum in HEK293, HeLa, and A549 cell culture media.

•  Lipid Mixture Solution (Catalog #LM-200):

This 200x lipid mixture solution contains non-animal derived fatty acids and lipids, designed to improve cell growth in serum-free media.

 


PeproGrow-1 Serum-Free Cell Culture Supplement Kit Figures and Descriptions


Figure 19 

Western Blot images using PeproGrow-1 for protein production

A. Western blot for Human TLR-3 production using PeproGrow-1 Serum-Free Cell Culture Supplement Kit

B. Western blot for Human sDLL-1 production using PeproGrow-1 Serum-Free Cell Culture Supplement Kit


PeproGrow-1 Kit (SR-100 & LM-200)

Catalog #700-C100

     Serum Replacement Solution SR-100 
     Lipid Mixture Solution LM-200 

 



PeproGrow™ Media Products Chart

*Select media products have individual components available for purchase



 


Cell Culture Glossary

 

    Adherent Cell Culture Cells that form a single layer on an artificial substrate system in a medium, such as in a T-flask or a roller bottle. 
Adult Stem Cell: A stem cell in the adult system that is in an undifferentiated state and maintains the ability to differentiate, generally into a cell type from its tissue of origin.
Animal-Free Medium: The medium does not contain animal-derived components.
Aseptic Technique: Procedures that are performed under sterile conditions. This prevents the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cell culture or other laboratory culturing conditions. These procedures may also prevent cell cross-contamination.
B Basal Medium: An incomplete simple cell culturing medium that does not contain supplemental nutrients or growth factors.
Blastocyst: This is the early stage of an embryo composed of a fluid filled cavity, an inner cell mass, and an outer cell mass.  Embryonic stems cells are derived from the inner cell mass, while the outer cell mass forms the trophoblast.
C Cellular Differentiation: The process that occurs when a cell becomes specialized for a particular cell type and its function(s).
Chemically-Defined Medium: The chemicals contained in the medium are known and quantifiable. This medium does not contain yeast, animal or plant protein hydrolysates.
Complete Medium: All the necessary components/nutrients for the cell type are present for culturing.
D Defined Medium: This is also known as chemically-defined medium.  All the chemicals and quantities of the medium content are known.
G Growth Supplement: This is an additional supplement that PeproTech supplies with some media products and the researcher adds this to the medium upon use, which contains growth factors/proteins necessary for the particular cell type's growth. The addition of the growth supplement results in the complete medium.
H Human Embryonic Stem Cell: A pluripotent stem cell that is derived from the inner cell mass of a blastocyst and can differentiate into different cell types.
I Induced Pluripotent Stem Cell (iPSC):                                                                              A stem cell that has been reprogrammed from an adult cell and has the ability to differentiate into other cell types.
M Medium/Media (plural form of medium):                                                       The liquid solution or growth medium to grow and cultivate a particular cell type.
P Pluripotent Stem Cell: A stem cell that is derived from the inner cell mass of the blastocyst an can differentiate into different cell types.
Progenitor Cell: A cell that can differentiate, but cannot renew itself.  Generally, a progenitor cell is at a further stage of differentiation.
Protein-Free Medium: The medium does not contain protein, such as insulin,transferrin, albumin, and other protein growth factors. 
S Serum-Free Medium: The medium does not contain serum.
Suspension Cell Culture: Cells that are free floating in a medium, such as in a shaker flask on an orbital shaker, or a stirred tank bioreactor.
U Undefined Medium: The medium may contain ingredient(s), such as a yeast extract, and animal or plant protein hydrolysates, in which the mixture of the chemical reagents is in unknown proportions.
X Xeno-Free Medium: By some definitions xeno-free medium does not contain animal products, i.e., all the components are of non-animal origin. A xeno-free product may contain human-derived reagents.