PeproTech’s PeproGrow™ hESC Media products are comprised of complete, chemically-defined formulations designed for the feeder-free expansion and maintenance of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). Intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (Nanog+/Lin28+/Tra-1-60+/SSEA1-/SSEA4+/Oct4+), these formulations demonstrate less than 15% spontaneous differentiation as indicated by immunofluorescent staining and flow cytometry. These proprietary formulations were designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University. Each PeproGrow™ hESC Medium Kit includes a bottle of basal medium and a separate, lyophilized component of PeproTech’s recombinant growth factors. Additional companion products are available separately and include the Animal-Free Human Vitronectin Matrix and Buffer Kit (catalog number AF-VMB-220), and the Cell Passaging/Non-Enzymatic Detachment Buffer (catalog number CPD-125). PeproTech’s Animal-Free Human Vitronectin Matrix and Buffer Kit can be used following either a surface-coating or premix method.
PeproGrow™ hESC Medium Kit is an insulin-free formulation, in which insulin is replaced by a unique activator of similar pathways to eliminate possible interference during the measurement of insulin-production in subsequently differentiated cells.
PSC Immunophenotypes Are Maintained in PeproGrow hESC Medium
Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs) expanded in PeproGrow™ hESC Medium maintain an Oct4+/SSEA4+/Nanog+/Tra-1-60+/SSEA1- phenotype as shown by flow cytometry (A) and immunofluorescent microscopy (B).
Pluripotent Stem Cells Grow and Proliferate Rapidly in PeproGrow™ hESC Medium
H1 Human ES Cells transduced with turboGFP-Neo driven by an Oct4 promoter and expanded on Matrigel proliferate rapidly when grown in PeproGrow™ hESC Medium.