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Antigen Affinity Purified Polyclonal Antibodies

Anti-Human HB-EGF

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Product Details

Catalogue Number: 500-P329
Anti-Human HB-EGF

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HB-EGF. Anti-Human HB-EGF-specific antibody was purified by affinity chromatography employing an immobilized Human HB-EGF matrix.

Immunogen: E.coli-derived, 9.7 kDa Recombinant Human HB-EGF (PeproTech Catalog# 100-47)

Sandwich ELISA: To detect Human HB-EGF by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Rabbit Anti-Human HB-EGF (500-P329BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human HB-EGF.

Rabbit Anti-Human HB-EGF Sandwich ELISA

Western Blot: To detect Human HB-EGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Rabbit Anti-Human HB-EGF Western Blot Reduced Rabbit Anti-Human HB-EGF Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

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