Growth Factors & Cytokines

Recombinant Human Adiponectin

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Product Details

Catalogue Number: 450-24
Description:
Recombinant Human Adiponectin

Adiponectin is an adipose-derived secreted protein containing 226 amino acid residues. It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein. The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes. Disruption of adiponectin in mice causes insulin resistance and neointimal formation. Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity. The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-α and class A scavenger receptor in macrophages). Recombinant Human Adiponectin is a multimeric glycoprotein containing amino acids Glu-19 to Asn-244 of the adiponectin precursor protein fused to an N-terminal histidine tag. Monomeric glycosylated adiponectin migrates at an apparent molecular weight of approximately 35.0 kDa by SDS PAGE analysis under reducing conditions.  The calculated molecular weight of Recombinant Human Adiponectin is 25.9 kDa.

Source: Hi-5 Insect cells

Synonyms: Acrp-30, GBP-28, APM-1, Gelatin-binding protein, AdipoQ

AA Sequence: RGHHHHHHHH ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN

Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 3.0-6.0 μg/ml.

Calculated Molecular Weight: 25.9 kDa

Accession Number: Q15848

Gene ID: 9370

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Spinnler, R
Title
The adipocytokine Nampt and its product NMN have no effect on beta-cell survival but potentiate glucose stimulated insulin secretion.
Citation
PLoS ONE; 8(1) pge54106
PubMed Id
First Author
Wijesekara, N
Title
Adiponectin-induced ERK and Akt phosphorylation protects against pancreatic beta cell apoptosis and increases insulin gene expression and secretion.
Citation
The Journal of Biological Chemistry; 285(44) pg33623-31
PubMed Id
First Author
Kirouac, D C
Title
Dynamic interaction networks in a hierarchically organized tissue.
Citation
Molecular Systems Biology; 6 pg417
PubMed Id