Animal-Free Recombinant Human EGF 5 ReviewsSubmit a Review Product Details Catalogue Number: AF-100-15 Description: EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds. *Animal-Free Recombinant Human EGF (Catalog Number AF-100-15) has replaced Recombinant Human EGF (Catalog Number 100-15) Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Epidermal Growth Factor, Urogastrone, URG AA Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 6.2 kDa Accession Number: P01133 Gene ID: 1950 crossreactivity: Chicken, Cow, Dog, Hamster, Horse, Human, Human + Hamster, Monkey, Mouse, Pig, Rabbit, Rat, Salamander, Snake, Tunicate, Zebra Fish References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cancer Cell Culture Immune System Inflammation Neurobiology Stem Cells & Differentiation Wound Healing Diabetes/Weight Regulation product.subtitle.recentcitations First Author Tang, T Title Protease Nexin I is a feedback regulator of EGF/PKC/MAPK/EGR1 signaling in breast cancer cells metastasis and stemness. Citation Cell Death & Disease; 10(9) pg649 PubMed Id 31501409 First Author Sommer, U Title Mechanisms of SOCS3 phosphorylation upon interleukin-6 stimulation. Contributions of Src- and receptor-tyrosine kinases. Citation The Journal of Biological Chemistry; 280(36) pg31478-88 PubMed Id 16000307 First Author Sparta, B Title Receptor Level Mechanisms Are Required for Epidermal Growth Factor (EGF)-stimulated Extracellular Signal-regulated Kinase (ERK) Activity Pulses. Citation The Journal of Biological Chemistry; 290(41) pg24784-92 PubMed Id 26304118 product.subtitle.productreviews Existing reviews Quality EGF for Organoid Culture one of best quality growth factors from peprotech, we are using for various organoid media. Consistent quality, very very cost effective than competitor suppliers, next day delivery. From: Hardik | Date: 8/14/2020 × Quality EGF for Organoid Culture one of best quality growth factors from peprotech, we are using for various organoid media. Consistent quality, very very cost effective than competitor suppliers, next day delivery. From: Hardik | Date: 8/14/2020 Close Constant quality for in vitro culture Using for in vitro culture. Constant quality. Also quick delivery. From: KOHSUKE | Date: 8/24/2021 × Constant quality for in vitro culture Using for in vitro culture. Constant quality. Also quick delivery. From: KOHSUKE | Date: 8/24/2021 Close EGF for primary cell culture I have used Peprotech human recombinant EGF and FGF for the culture of primary glioblastoma cells for several years now. Works well, no complaints and any questions I have had have been answered quickly and thoroughly by Peprotech customer service From: Kathleen | Date: 8/24/2021 × EGF for primary cell culture I have used Peprotech human recombinant EGF and FGF for the culture of primary glioblastoma cells for several years now. Works well, no complaints and any questions I have had have been answered quickly and thoroughly by Peprotech customer service From: Kathleen | Date: 8/24/2021 Close EGF, human basic recombinant, E. coli Using the EGF for 5 years now in our culture medium and are very satisfied ! Critical cells, but they grow very well with the EGF (and FGF) of Peprotech ! From: Arjan | Date: 8/27/2021 × EGF, human basic recombinant, E. coli Using the EGF for 5 years now in our culture medium and are very satisfied ! Critical cells, but they grow very well with the EGF (and FGF) of Peprotech ! From: Arjan | Date: 8/27/2021 Close Great recombinant protein Works very nicely in combination with FGF to keep mouse ESC-derived NPCs alive and proliferating in culture (at 10 ng/ml concentration). Highly recommend. From: Epp | Date: 8/31/2021 × Great recombinant protein Works very nicely in combination with FGF to keep mouse ESC-derived NPCs alive and proliferating in culture (at 10 ng/ml concentration). Highly recommend. From: Epp | Date: 8/31/2021 Close
