Recombinant Human Eotaxin (CCL11)

Recombinant Human Eotaxin (CCL11)

Product Details

Catalog Number: 300-21

Description:

Eotaxin is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-γ-stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and, along with Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung and in allergic reactions. Recombinant Human Eotaxin is an 8.3 kDa protein containing 74 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Source: E.coli

Synonyms: CCL11

AA Sequence: GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to chemoattract human peripheral blood eosinophils using a concentration range of 0.1-10.0 ng/ml.

Cross Reactivity:

Bacteria, Guinea Pig, Human , Human + Guinea Pig, Human + Hamster, Human + Mouse, Mouse, N/A, Rat

Research Interest

Country Of Origin: USA

Not for human use.

First Author

Title

Citation

Pubmed

Struyf, S

CD26/dipeptidyl-peptidase IV down-regulates the eosinophil chemotactic potency, but not the anti-HIV activity of human eotaxin by affecting its interaction with CC chemokine receptor 3.

Journal of immunology (Baltimore, Md. : 1950); 162(8) pg4903-9

15 Apr 1999

10202035
Show Usages

Zimmermann, N

CC chemokine receptor-3 undergoes prolonged ligand-induced internalization.

The Journal of Biological Chemistry; 274(18) pg12611-8

30 Apr 1999

10212240
Show Usages

Romagnani, P

Tryptase-chymase double-positive human mast cells express the eotaxin receptor CCR3 and are attracted by CCR3-binding chemokines.

The American Journal of Pathology; 155(4) pg1195-204

0 Oct 1999

10514402
Show Usages

Proudfoot, A E

Amino-terminally modified RANTES analogues demonstrate differential effects on RANTES receptors.

The Journal of Biological Chemistry; 274(45) pg32478-85

5 Nov 1999

10542293
Show Usages

Zhang, J

Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium.

Biology of Reproduction; 62(2) pg404-11

0 Feb 2000

10642580
Show Usages

Elsner, J

Differential activation of CC chemokine receptors by AOP-RANTES.

The Journal of Biological Chemistry; 275(11) pg7787-94

17 Mar 2000

10713092
Show Usages

Sabroe, I

A small molecule antagonist of chemokine receptors CCR1 and CCR3. Potent inhibition of eosinophil function and CCR3-mediated HIV-1 entry.

The Journal of Biological Chemistry; 275(34) pg25985-92

25 Aug 2000

10854442
Show Usages

White, J R

Identification of potent, selective non-peptide CC chemokine receptor-3 antagonist that inhibits eotaxin-, eotaxin-2-, and monocyte chemotactic protein-4-induced eosinophil migration.

The Journal of Biological Chemistry; 275(47) pg36626-31

24 Nov 2000

10969084
Show Usages

Martinelli, R

The CC chemokine eotaxin (CCL11) is a partial agonist of CC chemokine receptor 2b.

The Journal of Biological Chemistry; 276(46) pg42957-64

16 Nov 2001

11559700
Show Usages

Lee, J K

Truncation of NH2-terminal amino acid residues increases agonistic potency of leukotactin-1 on CC chemokine receptors 1 and 3.

The Journal of Biological Chemistry; 277(17) pg14757-63

26 Apr 2002

11832479
Show Usages

Stubbs, V E

Indomethacin causes prostaglandin D(2)-like and eotaxin-like selective responses in eosinophils and basophils.

The Journal of Biological Chemistry; 277(29) pg26012-20

19 Jul 2002

11980903
Show Usages

Bryan, S A

Responses of leukocytes to chemokines in whole blood and their antagonism by novel CC-chemokine receptor 3 antagonists.

American Journal of Respiratory and Critical Care Medicine; 165(12) pg1602-9

15 Jun 2002

12070060
Show Usages

Forssmann, U

n-Nonanoyl-CC chemokine ligand 14, a potent CC chemokine ligand 14 analogue that prevents the recruitment of eosinophils in allergic airway inflammation.

Journal of immunology (Baltimore, Md. : 1950); 173(5) pg3456-66

1 Sep 2004

15322211
Show Usages

Ferland, C

IL-16 activates plasminogen-plasmin system and promotes human eosinophil migration into extracellular matrix via CCR3-chemokine-mediated signaling and by modulating CD4 eosinophil expression.

Journal of immunology (Baltimore, Md. : 1950); 173(7) pg4417-24

1 Oct 2004

15383572
Show Usages

Hartnell, A

Identification of selective basophil chemoattractants in human nasal polyps as insulin-like growth factor-1 and insulin-like growth factor-2.

Journal of immunology (Baltimore, Md. : 1950); 173(10) pg6448-57

15 Nov 2004

15528386
Show Usages

Howard, O M

Autoantigens signal through chemokine receptors: uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate.

Blood; 105(11) pg4207-14

1 Jun 2005

15713799
Show Usages

Munitz, A

The inhibitory receptor IRp60 (CD300a) suppresses the effects of IL-5, GM-CSF, and eotaxin on human peripheral blood eosinophils.

Blood; 107(5) pg1996-2003

1 Mar 2006

16254138
Show Usages

Munitz, A

CD48 is an allergen and IL-3-induced activation molecule on eosinophils.

Journal of immunology (Baltimore, Md. : 1950); 177(1) pg77-83

1 Jul 2006

16785501
Show Usages

Ponte, A L

The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.

Stem cells (Dayton, Ohio); 25(7) pg1737-45

0 Jul 2007

17395768
Show Usages

Wise, E L

Small molecule receptor agonists and antagonists of CCR3 provide insight into mechanisms of chemokine receptor activation.

The Journal of Biological Chemistry; 282(38) pg27935-43

21 Sep 2007

17635911
Show Usages

Schratl, P

The role of the prostaglandin D2 receptor, DP, in eosinophil trafficking.

Journal of immunology (Baltimore, Md. : 1950); 179(7) pg4792-9

1 Oct 2007

17878378
Show Usages

Sebzda, E

Transcription factor KLF2 regulates the migration of naive T cells by restricting chemokine receptor expression patterns.

Nature Immunology; 9(3) pg292-300

0 Mar 2008

18246069
Show Usages

Goplen, N

A phosphosite screen identifies autocrine TGF-beta-driven activation of protein kinase R as a survival-limiting factor for eosinophils.

Journal of immunology (Baltimore, Md. : 1950); 180(6) pg4256-64

15 Mar 2008

18322238
Show Usages

Spiegl, N

Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid.

Blood; 112(9) pg3762-71

1 Nov 2008

18495959
Show Usages

Forssmann, U

Inhibition of CD26/dipeptidyl peptidase IV enhances CCL11/eotaxin-mediated recruitment of eosinophils in vivo.

Journal of immunology (Baltimore, Md. : 1950); 181(2) pg1120-7

15 Jul 2008

18606664
Show Usages

Sturm, E M

Prostaglandin E2 inhibits eosinophil trafficking through E-prostanoid 2 receptors.

Journal of immunology (Baltimore, Md. : 1950); 181(10) pg7273-83

15 Nov 2008

18981149
Show Usages

Takeda, A

CCR3 is a target for age-related macular degeneration diagnosis and therapy.

Nature; 460(7252) pg225-30

9 Jul 2009

19525930
Show Usages

Izhak, L

A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological activity of CCL2.

Journal of immunology (Baltimore, Md. : 1950); 183(1) pg732-9

1 Jul 2009

19535619
Show Usages

Clements, D

Role of the CXCR4/CXCL12 axis in lymphangioleiomyomatosis and angiomyolipoma.

Journal of immunology (Baltimore, Md. : 1950); 185(3) pg1812-21

1 Aug 2010

20585037
Show Usages

Yung, S C

Host chemokines bind to Staphylococcus aureus and stimulate protein A release.

The Journal of Biological Chemistry; 286(7) pg5069-77

18 Feb 2011

21138841
Show Usages

Pazdrak, K

Priming of eosinophils by GM-CSF is mediated by protein kinase CbetaII-phosphorylated L-plastin.

Journal of immunology (Baltimore, Md. : 1950); 186(11) pg6485-96

1 Jun 2011

21525390
Show Usages

Konya, V

A biased non-GÎ±i OXE-R antagonist demonstrates that GÎ±i protein subunit is not directly involved in neutrophil, eosinophil, and monocyte activation by 5-oxo-ETE.

Journal of immunology (Baltimore, Md. : 1950); 192(10) pg4774-82

15 May 2014

24733850
Show Usages

Lessel, D

The analysis of heterotaxy patients reveals new loss-of-function variants of GRK5.

Scientific Reports; 6 pg33231

13 Sep 2016

27618959
Show Usages

Singh, K

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific Reports; 7(1) pg4267

27 Jun 2017

28655871
Show Usages

Zhang, Y

A new antagonist for CCR4 attenuates allergic lung inflammation in a mouse model of asthma.

Scientific Reports; 7(1) pg15038

8 Nov 2017

29118379
Show Usages

Costa, A

Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer.

Cancer Cell; 33(3) pg463-479.e10

12 Mar 2018

29455927
Show Usages

Alenazi, Y

Genetically engineered two-warhead evasins provide a method to achieve precision targeting of disease-relevant chemokine subsets.

Scientific Reports; 8(1) pg6333

20 Apr 2018

29679010
Show Usages

Tiffany, H L

Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309.

The Journal of Experimental Medicine; 186(1) pg165-70

7 Jul 1997

9207005
Show Usages

Oravecz, T

Regulation of the receptor specificity and function of the chemokine RANTES (regulated on activation, normal T cell expressed and secreted) by dipeptidyl peptidase IV (CD26)-mediated cleavage.

The Journal of Experimental Medicine; 186(11) pg1865-72

1 Dec 1997

9382885
Show Usages

Bonecchi, R

Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s.

The Journal of Experimental Medicine; 187(1) pg129-34

5 Jan 1998

9419219
Show Usages

Campbell, J J

Chemokines and the arrest of lymphocytes rolling under flow conditions.

Science (New York, N.Y.); 279(5349) pg381-4

16 Jan 1998

9430588
Show Usages

Sallusto, F

Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes.

The Journal of Experimental Medicine; 187(6) pg875-83

16 Mar 1998

9500790
Show Usages

Hamada, T

Transendothelial migration of megakaryocytes in response to stromal cell-derived factor 1 (SDF-1) enhances platelet formation.

The Journal of Experimental Medicine; 188(3) pg539-48

3 Aug 1998

9687531
Show Usages

Bonecchi, R

Up-regulation of CCR1 and CCR3 and induction of chemotaxis to CC chemokines by IFN-gamma in human neutrophils.

Journal of immunology (Baltimore, Md. : 1950); 162(1) pg474-9

1 Jan 1999

9886422
Show Usages

Górski, P

Eotaxin but not MCP-3 induces eosinophil influx into nasal fluid in allergic patients.

Allergy; 57(6) pg519-28

0 Jun 2002

12028117
Show Usages

Böhm, E

11-Dehydro-thromboxane B2, a stable thromboxane metabolite, is a full agonist of chemoattractant receptor-homologous molecule expressed on TH2 cells (CRTH2) in human eosinophils and basophils.

The Journal of Biological Chemistry; 279(9) pg7663-70

27 Feb 2004

14668348
Show Usages

Górski, P

Eotaxin but not MCP-3 induces eosinophil influx into nasal fluid in allergic patients.

Allergy; 57(6) pg519-28

0 Jun 2002

12028117
Show Usages

Böhm, E

11-Dehydro-thromboxane B2, a stable thromboxane metabolite, is a full agonist of chemoattractant receptor-homologous molecule expressed on TH2 cells (CRTH2) in human eosinophils and basophils.

The Journal of Biological Chemistry; 279(9) pg7663-70

27 Feb 2004

14668348
Show Usages