Recombinant Human GDNF 3 ReviewsSubmit a Review Product Details Catalogue Number: 450-10 Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa. Source: E.coli Synonyms: Glial-Derived Neurotrophic Factor, ATF-1 AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 30.4 kDa Accession Number: P39905 Gene ID: 2668 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Bird, Frog, Human, Human + Hamster, Human + Mouse, Human + Rat, Human + Virus, Monkey, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily product.subtitle.recentcitations First Author Mesci, P Title SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with Sofosbuvir. Citation PLoS Biology; 20(11) pge3001845 PubMed Id 36327326 First Author Sabate-Soler, S Title Microglia integration into human midbrain organoids leads to increased neuronal maturation and functionality. Citation Glia; 70(7) pg1267-1288 PubMed Id 35262217 First Author Lee, Y Title Mitochondrial genome mutations and neuronal dysfunction of induced pluripotent stem cells derived from patients with Alzheimer's disease. Citation Cell Proliferation; 55(7) pge13274 PubMed Id 35698260 product.subtitle.productreviews Existing reviews High quality product Used for human stem cells. Awesome product! From: Danielle | Date: 8/26/2020 × High quality product Used for human stem cells. Awesome product! From: Danielle | Date: 8/26/2020 Close Cortical Neuron Differentiation Very consistent for culturing human stem cells From: Danielle | Date: 8/24/2021 × Cortical Neuron Differentiation Very consistent for culturing human stem cells From: Danielle | Date: 8/24/2021 Close iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. From: Mandi | Date: 8/25/2021 × iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. From: Mandi | Date: 8/25/2021 Close
