Growth Factors & Cytokines

Recombinant Human GM-CSF

Product Details

Catalogue Number: 300-03
Description:

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

Source: E.coli

Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α

AA Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 14.6 kDa

Accession Number: P04141

Gene ID: 1437

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Nardelli, B
Title
Characterization of the signal transduction pathway activated in human monocytes and dendritic cells by MPIF-1, a specific ligand for CC chemokine receptor 1.
Citation
Journal of immunology (Baltimore, Md. : 1950); 162(1) pg435-44
PubMed Id
First Author
Goff, J P
Title
Influence of cytokines on the growth kinetics and immunophenotype of daughter cells resulting from the first division of single CD34(+)Thy-1(+)lin- cells.
Citation
Blood; 92(11) pg4098-107
PubMed Id
First Author
Lee, K H
Title
Functional dissociation between local and systemic immune response during anti-melanoma peptide vaccination.
Citation
Journal of immunology (Baltimore, Md. : 1950); 161(8) pg4183-94
PubMed Id

product.subtitle.productreviews

Existing reviews
Consistent
This product has been working pretty well for human primary myeloid cell assays with consistent results.
From:  Noriyuki | Date:  7/8/2020