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Growth Factors & Cytokines

Recombinant Human HGF (HEK293 derived)

Product Details

Catalog Number: 100-39H
Description:

HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 697 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. Recombinant Human HGF, sourced from HEK293 cells, is a 79.4 kDa polypeptide consisting of 695 amino acid residues. As a result of glycosylation, Recombinant Human HGF migrates with an apparent molecular mass of approximately 68-85 kDa by SDS-PAGE gel, under non-reducing conditions.

Source: HEK293 cells

Synonyms: Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA)

AA Sequence:

alpha chain: KRRNTIHEFK KSAKTTLIKI DPALKIKTKK VNTADQCANR CTRNKGLPFT CKAFVFDKAR KQCLWFPFNS MSSGVKKEFG HEFDLYENKD YIRNCIIGKG RSYKGTVSIT KSGIKCQPWS SMIPHEHSFL PSSYRGKDLQ ENYCRNPRGE EGGPWCFTSN PEVRYEVCDI PQCSEVECMT CNGESYRGLM DHTESGKICQ RWDHQTPHRH KFLPERYPDK GFDDNYCRNP DGQPRPWCYT LDPHTRWEYC AIKTCADNTM NDTDVPLETT ECIQGQGEGY RGTVNTIWNG IPCQRWDSQY PHEHDMTPEN FKCKDLRENY CRNPDGSESP WCFTTDPNIR VGYCSQIPNC DMSHGQDCYR GNGKNYMGNL SQTRSGLTCS MWDKNMEDLH RHIFWEPDAS KLNENYCRNP DDDAHGPWCY TGNPLIPWDY CPISRCEGDT TPTIVNLDHP VISCAKTKQL R

beta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.

Calculated Molecular Weight: 79.4 kDa

Accession Number: P14210

Gene ID: 3082

Cross Reactivity Cited in References:
Country Of Origin: USA

Not for human use.

Research Interest

Product Reviews

Existing reviews
Reliable product, reproducible results, fast shipping!
I use this product for differentiating human iPSCs, and my differentiations have been highly reproducible using HGF from Peprotech. Plus, the shipping is fast, and I appreciate the product inserts with clear storage recommendations. Overall, great experience with this product, and I will continue to order!
From:  Kendall | Date:  8/26/2020
Fast shipping, consistent quality
I use peprotech small molecules for all my iPSC differentiations and I am very happy with the results. Shipping is very fast too.
From:  Aylin | Date:  8/26/2020
High quality and good price
This product worked well for my project.  The price is reasonable and lower than most of vendors.
From:  Jin | Date:  8/26/2020