Growth Factors & Cytokines

Recombinant Human IGF-I LR3

Product Details

Catalogue Number: 100-11R3
Description:
Recombinant Human IGF-I LR3

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. IGF-I LR3 is a recombinant analog of human IGF-I comprised of the complete IGF-I sequence, with an Arginine substitution for the third position Glutamic acid, and a 13 amino acid length N terminus peptide extension. Specifically engineered for higher biological potency in vitro, IGF-I LR3 has an increased half-life and a binding aversion to native proteins within the body that make it ideal for both research and large-scale culturing. Recombinant Human IGF-I LR3 is a 9.1 kDa, single, non-glycosylated polypeptide chain containing 83 amino acid residues.

Source: E.coli

Synonyms: Long R3 IGF-I, Insulin-like Growth Factor-I, Somatamedin C, IGF-IA

AA Sequence: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

Calculated Molecular Weight: 9.1 kDa

Accession Number: P05019

Gene ID: 3479

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Sharma, P
Title
Exosomes regulate neurogenesis and circuit assembly.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 116(32) pg16086-16094
PubMed Id
First Author
Carrió, M
Title
Reprogramming Captures the Genetic and Tumorigenic Properties of Neurofibromatosis Type 1 Plexiform Neurofibromas.
Citation
Stem Cell Reports; 12(2) pg411-426
PubMed Id
First Author
Wu, J
Title
Interspecies Chimerism with Mammalian Pluripotent Stem Cells.
Citation
Cell; 168(3) pg473-486.e15
PubMed Id

product.subtitle.productreviews

Existing reviews
IGF-1
This products works great to help differentiation of neurons and works consistently well.
From:  Aala | Date:  8/26/2020