Recombinant Human IL-10 1 ReviewsSubmit a Review Product Details Catalogue Number: 200-10 Description: IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells. Recombinant Human IL-10 is an 18.6 kDa protein of 161 amino acid residues. Source: E.coli Synonyms: B-TCGF, CSIF, TGIF AA Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 18.6 kDa Accession Number: P22301 Gene ID: 3586 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Bacteria, Human, Human + Virus, Monkey, Mouse, Virus References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Cancer Immune System Inflammation Neurobiology Stem Cells & Differentiation Allergy Transplantation product.subtitle.recentcitations First Author George, S Title Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned media. Citation STAR Protocols; 3(4) pg101666 PubMed Id 36125932 First Author Miki, S Title S-1-Propenylcysteine promotes IL-10-induced M2c macrophage polarization through prolonged activation of IL-10R/STAT3 signaling. Citation Scientific Reports; 11(1) pg22469 PubMed Id 34789834 First Author Aguilar-Bretones, M Title Seasonal coronavirus-specific B cells with limited SARS-CoV-2 cross-reactivity dominate the IgG response in severe COVID-19. Citation The Journal of Clinical Investigation; 131(21) PubMed Id 34499051 product.subtitle.productreviews Existing reviews Good quality This cytokine works well for our in vitro T cell studies. High quality and consistent results between batches. From: Jacqueline | Date: 8/3/2021 × Good quality This cytokine works well for our in vitro T cell studies. High quality and consistent results between batches. From: Jacqueline | Date: 8/3/2021 Close
