Growth Factors & Cytokines

Recombinant Human IL-10

Product Details

Catalogue Number: 200-10
Description:

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes.  IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α.  Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.  Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells.  Recombinant Human IL-10 is an 18.6 kDa protein of 161 amino acid residues.

Source: E.coli

Synonyms: B-TCGF, CSIF, TGIF

AA Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 18.6 kDa

Accession Number: P22301

Gene ID: 3586

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Hirohata, S
Title
Human Th1 responses driven by IL-12 are associated with enhanced expression of CD40 ligand.
Citation
Clinical and Experimental Immunology; 115(1) pg78-85
PubMed Id
First Author
Gorczynski, R M
Title
Analysis of cytokine production and V beta T-cell receptor subsets in irradiated recipients receiving portal or peripheral venous reconstitution with allogeneic bone marrow cells, with or without additional anti-cytokine monoclonal antibodies.
Citation
Immunology; 93(2) pg221-9
PubMed Id
First Author
Kucharzik, T
Title
IL-4, IL-10 and IL-13 down-regulate monocyte-chemoattracting protein-1 (MCP-1) production in activated intestinal epithelial cells.
Citation
Clinical and Experimental Immunology; 111(1) pg152-7
PubMed Id

product.subtitle.productreviews

Existing reviews
Good quality
This cytokine works well for our in vitro T cell studies. High quality and consistent results between batches.
From:  Jacqueline | Date:  8/3/2021