Growth Factors & Cytokines

Recombinant Human IL-4

Product Details

Catalogue Number: 200-04
Description:

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant Human IL-4 is a 15.1 kDa globular protein containing 130 amino acid residues.

Source: E.coli

Synonyms: BCGF, BCDF, B cell stimulating factor (BSF-1)

AA Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 15.1 kDa

Accession Number: P05112

Gene ID: 3565

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Hirohata, S
Title
Human Th1 responses driven by IL-12 are associated with enhanced expression of CD40 ligand.
Citation
Clinical and Experimental Immunology; 115(1) pg78-85
PubMed Id
First Author
Morgan, J W
Title
Differential regulation of gene transcription in subpopulations of human B lymphocytes by vitamin D3.
Citation
Endocrinology; 140(1) pg381-91
PubMed Id
First Author
Nardelli, B
Title
Characterization of the signal transduction pathway activated in human monocytes and dendritic cells by MPIF-1, a specific ligand for CC chemokine receptor 1.
Citation
Journal of immunology (Baltimore, Md. : 1950); 162(1) pg435-44
PubMed Id

product.subtitle.productreviews

Existing reviews
hIL4
Good product. Used for in vitro culture to derive BMDCs.
From:  Kevin | Date:  8/26/2020
Great for APCs
Used to differentiate APCs, works very consistently
From:  Karma | Date:  8/26/2020
MDSCs
Used to differentiate PBMCs into MDSCs. Works well.
From:  Max | Date:  8/27/2020