Recombinant Human LIGHT 0 ReviewsSubmit a Review Product Details Catalogue Number: 310-09B Description: LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTβR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to activate NF-κB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells. The human LIGHT gene encodes for a 240 amino acid type II transmembrane protein containing a 37 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 182 amino acid extracellular domain. Recombinant Human LIGHT has a calculated mass of 19.3 kDa, containing 177 amino acid residues. Due to glycosylation, LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions. *Human LIGHT(Catalog Number 310-09B) has replaced Human LIGHT(Catalog Number 310-09) Source: Hi-5 Insect cells Synonyms: TNFSF14, HVEM-L AA Sequence: RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce IL-8 secretion in human BEAS-2B cells. Calculated Molecular Weight: 19.3 kDa Accession Number: O43557 Gene ID: 8740 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 15μg is the largest vial size for this product. Larger sizes supplied as multiple 15μg vials. PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Cancer Immune System Inflammation
