Growth Factors & Cytokines

Recombinant Human M-CSF

Product Details

Catalogue Number: 300-25
Description:

M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Human M-CSF is a 36.8 kDa homodimeric protein consisting of two 159 amino acid polypeptide subunits.

Source: E.coli

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM

AA Sequence (monomer): MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 36.8 kDa

Accession Number: P09603

Gene ID: 1435

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Naqvi, A R
Title
miR-24, miR-30b and miR-142-3p interfere with antigen processing and presentation by primary macrophages and dendritic cells.
Citation
Scientific Reports; 6 pg32925
PubMed Id
First Author
Park, K
Title
Monocyte-derived macrophage assisted breast cancer cell invasion as a personalized, predictive metric to score metastatic risk.
Citation
Scientific Reports; 5 pg13855
PubMed Id
First Author
Kakkanat, A
Title
The role of H4 flagella in Escherichia coli ST131 virulence.
Citation
Scientific Reports; 5 pg16149
PubMed Id

product.subtitle.productreviews

Existing reviews
Great Product
It is a great product for my study. I had used this product in several cell-line experiments including primary cell lines.
From:  Kanagavel | Date:  7/22/2020
Perfect for macrophages
M-CSF is wonderful for macrophage tissue culture.
From:  Heather | Date:  8/3/2021
Best MCSF on the market
My lab switched to Peprotech a few years ago for human MCSF and we will never go back. We generate human macrophages with this product and have only had consistent and great results. It's also competively priced for the quantity and so I'd definitely recommend it.
From:  Emilio | Date:  8/4/2021