Growth Factors & Cytokines

Recombinant Human PDGF-BB

Product Details

Catalogue Number: 100-14B
Description:

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant Human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two β chains (218 total amino acids). .

Source: E.coli

Synonyms: Platelet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

AA Sequence (monomer): SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells.The expected ED50 for this effect is 1.0-3.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 24.3 kDa

Accession Number: P01127

Gene ID: 5155

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chiarugi, P
Title
Reactive oxygen species as essential mediators of cell adhesion: the oxidative inhibition of a FAK tyrosine phosphatase is required for cell adhesion.
Citation
The Journal of Cell Biology; 161(5) pg933-44
PubMed Id
First Author
Haag, D
Title
H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model.
Citation
Cancer Cell; 39(3) pg407-422.e13
PubMed Id
First Author
Yang, L
Title
Phosphorylation of p68 RNA helicase plays a role in platelet-derived growth factor-induced cell proliferation by up-regulating cyclin D1 and c-Myc expression.
Citation
The Journal of Biological Chemistry; 282(23) pg16811-9
PubMed Id

product.subtitle.productreviews

Existing reviews
high quality and low price
We have used this product for in vitro smooth muscle cell differentiation from human IPSCs for more than 4 years, which contributes to several high quality publication  such as Circulation, ATVB, and Nature Comm. Compare to other's suppliers product, this one has high quality as well as reasonable price
From:  Ping | Date:  8/3/2021