Recombinant Human Persephin 0 ReviewsSubmit a Review Product Details Catalogue Number: 450-12 Description: Persephin is a disulfide-linked, homodimeric, neurotrophic factor structurally related to GDNF, artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR α (α1-α4) receptors. The GFRα4 was first identified in chicken, and was later shown to be the preferential binding subunit for persephin. Persephin promotes the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve oxotomy, and, like GDNF, promotes ureteric bud branching. However, in contrast to GDNF and neurturin, persephin does not support the survival of peripheral neurons. Recombinant Human Persephin is a disulfide-linked homodimer, composed of two 10.4 kDa polypeptide chains (194 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 64) is used for inter-chain disulfide bridging, and the others are involved in the intramolecular ring formation known as the cysteine knot configuration. Source: E.coli Synonyms: PSP, PSPN AA Sequence (monomer): RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0 ng/ml. Human Persephin binds to mammalian GFRa4 with the Kd of 100pM. Other members of the GDNF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFRa4. Calculated Molecular Weight: 20.8 kDa Accession Number: O60542 Gene ID: 5623 crossreactivity: Human, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily product.subtitle.recentcitations First Author Yang, J Title The mouse soluble GFRalpha4 receptor activates RET independently of its ligand persephin. Citation Oncogene; 26(26) pg3892-8 PubMed Id 17213820 First Author Lindahl, M Title Human glial cell line-derived neurotrophic factor receptor alpha 4 is the receptor for persephin and is predominantly expressed in normal and malignant thyroid medullary cells. Citation The Journal of Biological Chemistry; 276(12) pg9344-51 PubMed Id 11116144 First Author Hanson, M G Title Cyclic AMP elevation is sufficient to promote the survival of spinal motor neurons in vitro. Citation The Journal of neuroscience : the official journal of the Society for Neuroscience; 18(18) pg7361-71 PubMed Id 9736656