Growth Factors & Cytokines

Recombinant Human R-Spondin-1

Product Details

Catalogue Number: 120-38
Description:

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/β-catenin signaling pathway. Recombinant Human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues.  Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.

Source: CHO cells

Synonyms: Roof plate-specific spondin-1, RSPO1

AA Sequence: SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 μg/ml.

Calculated Molecular Weight: 26.7 kDa

Accession Number: Q2MKA7

Gene ID: 284654

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Koikawa, K
Title
Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.
Citation
Cell; 184(18) pg4753-4771.e27
PubMed Id
First Author
Cheung, V C
Title
Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua.
Citation
Cell Reports; 35(7) pg109138
PubMed Id
First Author
Karkampouna, S
Title
Patient-derived xenografts and organoids model therapy response in prostate cancer.
Citation
Nature Communications; 12(1) pg1117
PubMed Id

product.subtitle.productreviews

Existing reviews
Perfect for organoid culture
My lung organoids grow well consistently even after passaging it couple of times and there were no  contamination issues.
From:  Eun Joo | Date:  7/8/2020
Good for organoid
This is good for my primary lung organoid culture.
From:  Jun | Date:  7/21/2020
High quality and good price
This product worked well for my project.  The price is reasonable and lower than most of vendors.
From:  Jin | Date:  8/26/2020
Excellent qualiity
We have been using recombinant human R-spondin-1 for over 6 years and have been absolutely satisfied with the quality, purity, and performance!
From:  Candace | Date:  8/3/2021