Recombinant Human SCF 2 ReviewsSubmit a Review Product Details Catalog Number: 300-07 Description: SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Source: E.coli Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor AA Sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 18.4 kDa Accession Number: P21583 Gene ID: 4254 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Bacteria, Human, Human + Hamster, Human + Mouse, Human + Virus, Leech, Monkey, Mouse, Pig, Rabbit, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation Recent Citations First Author Li, Y Title Modulation of WNT, Activin/Nodal, and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation During Human iPSC Differentiation. Citation Stem cells (Dayton, Ohio); 41(7) pg685-697 PubMed ID 37220178 First Author Chen, W Title Reprogramming of human peripheral blood mononuclear cells into induced mesenchymal stromal cells using non-integrating vectors. Citation Communications Biology; 6(1) pg393 PubMed ID 37041280 First Author Thomas, G E Title The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness. Citation Nature Cell Biology; 24(6) pg872-884 PubMed ID 35668135 Product Reviews Existing reviews Most reliable As a huge lab at UCLA, we have been using Peprotech products consistently, this has been a long history of buying hSCF, and the quality has been consistent this whole time. From: Marlene | Date: 7/8/2020 × Most reliable As a huge lab at UCLA, we have been using Peprotech products consistently, this has been a long history of buying hSCF, and the quality has been consistent this whole time. From: Marlene | Date: 7/8/2020 Close Optimal performance for CD34+ cell culture Used for human CD34+ in vitro expansion culture, Peprotech hSCF performs as expected and yields optimal HSPC expansion while retaining phenotypic and functional HSC features. From: Gabriele | Date: 8/3/2021 × Optimal performance for CD34+ cell culture Used for human CD34+ in vitro expansion culture, Peprotech hSCF performs as expected and yields optimal HSPC expansion while retaining phenotypic and functional HSC features. From: Gabriele | Date: 8/3/2021 Close
