Recombinant Human TGF-β1 (HEK293 derived) 12 ReviewsSubmit a Review Product Details Catalogue Number: 100-21 Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors. Recombinant Human TGF-β1 derived from HEK293 cells is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond. Source: HEK293 cells Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor AA Sequence (monomer): ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 25 kDa Accession Number: P01137 Gene ID: 7040 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Chicken, Cow, Dog, Donkey, Frog, Human, Human + Mouse, Mink, Monkey, Mouse, Mouse + Virus, Pig, Rabbit, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Apoptosis Bone, Skeletal, Cartilage Cancer Cell Culture Immune System Inflammation Neurobiology Stem Cells & Differentiation TGF-β Superfamily Wound Healing Allergy product.subtitle.recentcitations First Author Abe, S Title Differences in the stemness characteristics and molecular markers of distinct human oral tissue neural crest-derived multilineage cells. Citation Cell Proliferation; 55(10) pge13286 PubMed Id 35716037 First Author Park, J Title Multi-Omics-Based Autophagy-Related Untypical Subtypes in Patients with Cerebral Amyloid Pathology. Citation Advanced science (Weinheim, Baden-Wurttemberg, Germany); 9(23) pge2201212 PubMed Id 35694866 First Author Otake-Kasamoto, Y Title Lysophosphatidylserines derived from microbiota in Crohn's disease elicit pathological Th1 response. Citation The Journal of Experimental Medicine; 219(7) PubMed Id 35608941 product.subtitle.productreviews Existing reviews Good quality price for the best results We have been using this reagent for years for creating neo cartilage from our chondrocytes and MSCs with very satisfactory results! I'd completely recommend it. From: Alejandro | Date: 7/9/2020 × Good quality price for the best results We have been using this reagent for years for creating neo cartilage from our chondrocytes and MSCs with very satisfactory results! I'd completely recommend it. From: Alejandro | Date: 7/9/2020 Close For Treg induction Very good for experiments with induced regulatory T cells, enables efficient induction of human Treg at 10-20ng/ml. Consistent across lots. From: Julian | Date: 7/10/2020 × For Treg induction Very good for experiments with induced regulatory T cells, enables efficient induction of human Treg at 10-20ng/ml. Consistent across lots. From: Julian | Date: 7/10/2020 Close Good for EMT induction We have been using it for years to induce EMT in human primary small airway epithelial cells. From: Jun | Date: 7/21/2020 × Good for EMT induction We have been using it for years to induce EMT in human primary small airway epithelial cells. From: Jun | Date: 7/21/2020 Close Good! Worked as Described! From: Lillian | Date: 7/21/2020 × Good! Worked as Described! From: Lillian | Date: 7/21/2020 Close Good and cheap Works quite well in organoid cultures and it's for a very reasonable price. From: Melissa | Date: 7/29/2020 × Good and cheap Works quite well in organoid cultures and it's for a very reasonable price. From: Melissa | Date: 7/29/2020 Close Works well to induce collagen and GAG production in neocartilage Our group has used this product for years and we are always pleased with the outcome in our systems. Batch to batch variation is very low and works consistently in culturing neocartilage with the intent to repair focal cartilage defects. From: Ryan | Date: 8/21/2020 × Works well to induce collagen and GAG production in neocartilage Our group has used this product for years and we are always pleased with the outcome in our systems. Batch to batch variation is very low and works consistently in culturing neocartilage with the intent to repair focal cartilage defects. From: Ryan | Date: 8/21/2020 Close Good quality product Thank you for providing us good quality cytokines with resonable price and fast delivery. Appreciate your service during Covid times. Quality of the product is excellent. From: Deepti | Date: 8/31/2020 × Good quality product Thank you for providing us good quality cytokines with resonable price and fast delivery. Appreciate your service during Covid times. Quality of the product is excellent. From: Deepti | Date: 8/31/2020 Close Quality Growth Factor for mesenchymal (EMT) signaling I used for Organoid Culture with mesenchymal signaling.Needed for squamous type of organoid growth. From: Hardik | Date: 8/31/2020 × Quality Growth Factor for mesenchymal (EMT) signaling I used for Organoid Culture with mesenchymal signaling.Needed for squamous type of organoid growth. From: Hardik | Date: 8/31/2020 Close good for pro-inflammatory and pro-fibrotic cocktails Using this at 3 ng/ml or 5 ng/ml promoted some inflammatory and fibrosis pathways we were investigating. This was very helpful when we needed to evaluate molecular mechanisms of our drug targets. From: Vikram | Date: 8/3/2021 × good for pro-inflammatory and pro-fibrotic cocktails Using this at 3 ng/ml or 5 ng/ml promoted some inflammatory and fibrosis pathways we were investigating. This was very helpful when we needed to evaluate molecular mechanisms of our drug targets. From: Vikram | Date: 8/3/2021 Close Easy in use, easy dissolved Fine product of Peprotech. We use it diluted - added freshly - in our diffentiation medium and are very content with the product From: Helen | Date: 8/9/2021 × Easy in use, easy dissolved Fine product of Peprotech. We use it diluted - added freshly - in our diffentiation medium and are very content with the product From: Helen | Date: 8/9/2021 Close Good and consistent We have successfully been using this product to activate fibroblasts in vitro with concentrations from 5 to 20 ng/mL. I highly reccomend it. From: Rita | Date: 8/23/2021 × Good and consistent We have successfully been using this product to activate fibroblasts in vitro with concentrations from 5 to 20 ng/mL. I highly reccomend it. From: Rita | Date: 8/23/2021 Close Chondrocyte responsive Worked well for my chondrocyte cell line, induced a good response From: Oliver | Date: 8/25/2021 × Chondrocyte responsive Worked well for my chondrocyte cell line, induced a good response From: Oliver | Date: 8/25/2021 Close
