Recombinant Human VEGF 165

Recombinant Human VEGF₁₆₅

Product Details

Catalog Number: 100-20

Description:

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant Human VEGF₁₆₅ is a 38.2 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Source: E.coli

Synonyms: Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF-A

AA Sequence (monomer): APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 38.2 kDa

Accession Number: P15692

Gene ID: 7422

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Cross Reactivity Cited in References:

Chicken, Cow, Gelatin Microparticles, Guinea Pig, Hamster, Horse, Human, Human + Hamster, Human + Mouse, Human + Rat, Monkey, Mouse, Mouse + Chicken, Pig, Rabbit, Rat, Sheep, tests, Trout, Tunicate, Zebra Fish

Country Of Origin: USA

Not for human use.

Research Interest

Angiogenesis/Cardiovascular

Stem Cells & Differentiation

Recent Citations

First Author

Kitajima, K

Title

An Interferon-γ/FLT3 Axis Positively Regulates Hematopoietic Progenitor Cell Expansion from Human Pluripotent Stem Cells.

Citation

Stem cells (Dayton, Ohio); 40(10) pg906-918

PubMed ID

35901509

First Author

Dhami, S P

Title

Breast cancer cells mediate endothelial cell activation, promoting von Willebrand factor release, tumor adhesion, and transendothelial migration.

Citation

Journal Of Thrombosis And Haemostasis : JTH; 20(10) pg2350-2365

PubMed ID

35722954

First Author

Hyun, J

Title

Notch1 promotes ordered revascularization through Semaphorin 3g modulation of downstream vascular patterning signalling factors.

Citation

The Journal of Physiology; 600(3) pg509-530

PubMed ID

34921404

Product Reviews

Existing reviews

Great for Endothelial Cell Differentiation

This is an excellent product with long-term stability if is stored correctly. I have been regularly using this product since 2017 for endothelial cell differentiation from human induced pluripotent cells (iPSCs). Thanks PeproTech for consistent quality product!

From: DIlip | Date: 7/13/2020

Excellent product for endothelial cells culture

I work since 2019 with human iPSC-derived endothelial cells, and the recombinant human VEGF 165 is mandatory for maintaining them in culture. VEGF 165 is an excellent product, it is stable for a long storage, easy to prepare and to use. Always receive it in time.I recommend this product. Thank you, PeproTech.

From: Amal | Date: 8/18/2021

Growth Factors & Cytokines