Recombinant Human WISP-1 0 ReviewsSubmit a Review Product Details Catalog Number: 120-18 Description: WISP-1 is a member of the CCN family of secreted, cysteine-rich regulatory proteins. It is expressed in the heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. WISP-1 is a β-catenin-regulated protein that can contribute to tumorigenesis, and has also been shown to play a role in bone development and fracture repair. Recombinant Human WISP-1 is a 38.1 kDa protein containing 345 amino acid residues. It is composed of four distinct structural domains (modules): the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain. Source: E.coli Synonyms: WNT-1 inducible signaling pathway protein-1, CCN4, Wnt-1-induced secreted protein AA Sequence: TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 µg/ml. Calculated Molecular Weight: 38.1 kDa Accession Number: O95388 Gene ID: 8840 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Bone, Skeletal, Cartilage Cancer Immune System Neurobiology Stem Cells & Differentiation Recent Citations First Author Maeda, A Title WNT1-induced Secreted Protein-1 (WISP1), a Novel Regulator of Bone Turnover and Wnt Signaling. Citation The Journal of Biological Chemistry; 290(22) pg14004-18 PubMed ID 25864198 First Author Venkatachalam, K Title WISP1, a pro-mitogenic, pro-survival factor, mediates tumor necrosis factor-alpha (TNF-alpha)-stimulated cardiac fibroblast proliferation but inhibits TNF-alpha-induced cardiomyocyte death. Citation The Journal of Biological Chemistry; 284(21) pg14414-27 PubMed ID 19339243 First Author Colston, J T Title Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor. Citation American journal of physiology. Heart and circulatory physiology; 293(3) pgH1839-46 PubMed ID 17616748
