Animal-Free Recombinant Human/Murine/Rat BMP-2 (E.coli derived) 0 ReviewsSubmit a Review Product Details Catalog Number: AF-120-02 Description: BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis, and is expressed in a variety of tissues, including lung, spleen, brain, liver, prostate, ovary, and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant Human/Murine/Rat BMP-2 is a 26.0 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Bone Morphogenetic Protein-2, BMP-2A AA Sequence (monomer): MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml. Calculated Molecular Weight: 26 kDa Accession Number: P12643 Gene ID: 650 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Apoptosis Bone, Skeletal, Cartilage Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily Recent Citations First Author Duan, X Title An airway organoid-based screen identifies a role for the HIF1α-glycolysis axis in SARS-CoV-2 infection. Citation Cell Reports; 37(6) pg109920 PubMed ID 34731648 First Author Pous, L Title Fibrinogen induces neural stem cell differentiation into astrocytes in the subventricular zone via BMP signaling. Citation Nature Communications; 11(1) pg630 PubMed ID 32005867 First Author Shortt, C Title A Hyaluronan-binding Peptide (P15-1) Reduces Inflammatory and Catabolic Events in IL-1β-treated Human Articular Chondrocytes. Citation Scientific Reports; 10(1) pg1441 PubMed ID 31996703
