Growth Factors & Cytokines

Recombinant Murine Flt3-Ligand

Product Details

Catalogue Number: 250-31L
Description:

Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand has no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant Murine Flt3-Ligand is a soluble 18.6 kDa protein consisting of 163 amino acid residues.

Source: E.coli

Synonyms: Fms-related tyrosine kinase 3 ligand, FltL

AA Sequence: MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. The expected ED50 for this effect is 5.0 – 8.0 ng/ml.

Calculated Molecular Weight: 18.6 kDa

Accession Number: P49772

Gene ID: 14256

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chiale, C
Title
Virus-based vaccine vectors with distinct replication mechanisms differentially infect and activate dendritic cells.
Citation
NPJ Vaccines; 6(1) pg138
PubMed Id
First Author
Gerosa, R C
Title
CXCL12-abundant reticular cells are the major source of IL-6 upon LPS stimulation and thereby regulate hematopoiesis.
Citation
Blood Advances; 5(23) pg5002-5015
PubMed Id
First Author
Zong, L
Title
NAD+ augmentation with nicotinamide riboside improves lymphoid potential of Atm-/- and old mice HSCs
Citation
NPJ Aging And Mechanisms Of Disease; 7(1) pg25
PubMed Id

product.subtitle.productreviews

Existing reviews
Recombinant Murin Flt3-Ligand
We use this product in our murine media. Upon arrival we make stock dilutions. They keep well in -20 until we are ready to prep more media
From:  Nichole | Date:  8/26/2020