Recombinant Murine Flt3-Ligand 1 ReviewsSubmit a Review Product Details Catalogue Number: 250-31L Description: Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand has no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant Murine Flt3-Ligand is a soluble 18.6 kDa protein consisting of 163 amino acid residues. Source: E.coli Synonyms: Fms-related tyrosine kinase 3 ligand, FltL AA Sequence: MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. The expected ED50 for this effect is 5.0 – 8.0 ng/ml. Calculated Molecular Weight: 18.6 kDa Accession Number: P49772 Gene ID: 14256 crossreactivity: Human, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cancer Cell Culture Immune System Stem Cells & Differentiation product.subtitle.recentcitations First Author Singh Rawat, B Title Methionine- and Choline-Deficient Diet Identifies an Essential Role for DNA Methylation in Plasmacytoid Dendritic Cell Biology. Citation Journal of immunology (Baltimore, Md. : 1950); 208(4) pg881-897 PubMed Id 35101891 First Author Anderson, D A Title Transition from cMyc to L-Myc during dendritic cell development coordinated by rising levels of IRF8. Citation The Journal of Experimental Medicine; 219(2) PubMed Id 34958351 First Author Westermann, S Title Siglec-F Promotes IL-33-Induced Cytokine Release from Bone Marrow-Derived Eosinophils Independently of the ITIM and ITIM-like Motif Phosphorylation. Citation Journal of immunology (Baltimore, Md. : 1950); 208(3) pg732-744 PubMed Id 34996839 product.subtitle.productreviews Existing reviews Recombinant Murin Flt3-Ligand We use this product in our murine media. Upon arrival we make stock dilutions. They keep well in -20 until we are ready to prep more media From: Nichole | Date: 8/26/2020 × Recombinant Murin Flt3-Ligand We use this product in our murine media. Upon arrival we make stock dilutions. They keep well in -20 until we are ready to prep more media From: Nichole | Date: 8/26/2020 Close
