Recombinant Murine IL-12 p70 2 ReviewsSubmit a Review Product Details Catalogue Number: 210-12 Description: IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues). Source: CHO cells Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF AA Sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Calculated Molecular Weight: 75 kDa Accession Number: p35: P43431 p40: P43432 Gene ID: p35: 16159 p40: 16160 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 1mg will be provided as 2x500μg. crossreactivity: Cow + Mouse, Human, Mouse, References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Angiogenesis/Cardiovascular Apoptosis Cancer Immune System Inflammation Neurobiology Stem Cells & Differentiation Allergy Transplantation product.subtitle.recentcitations First Author Karagiannis, F Title Impaired ketogenesis ties metabolism to T cell dysfunction in COVID-19. Citation Nature; 609(7928) pg801-807 PubMed Id 35901960 First Author Chang, J Title ICOS-Deficient Regulatory T Cells Can Prevent Spontaneous Autoimmunity but Are Impaired in Controlling Acute Inflammation. Citation Journal of immunology (Baltimore, Md. : 1950); 209(2) pg301-309 PubMed Id 35760518 First Author Otake-Kasamoto, Y Title Lysophosphatidylserines derived from microbiota in Crohn's disease elicit pathological Th1 response. Citation The Journal of Experimental Medicine; 219(7) PubMed Id 35608941 product.subtitle.productreviews Existing reviews Great product We have been using this cytokine for splenic NK cells in different publications for the past 5 years. Great consistency! From: Jin | Date: 7/21/2020 × Great product We have been using this cytokine for splenic NK cells in different publications for the past 5 years. Great consistency! From: Jin | Date: 7/21/2020 Close Great cytokine to make CD4 Th1 cells We have been using peprotech cytokines for years now. This cytokine has consistently worked for us to differentiate mouse CD4 Th1 cells. From: Chirag | Date: 8/31/2020 × Great cytokine to make CD4 Th1 cells We have been using peprotech cytokines for years now. This cytokine has consistently worked for us to differentiate mouse CD4 Th1 cells. From: Chirag | Date: 8/31/2020 Close
