Recombinant Murine IL-12 p70 2 ReviewsSubmit a Review Product Details Catalogue Number: 210-12 Description: IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues). Source: CHO cells Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF AA Sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Calculated Molecular Weight: 75 kDa Accession Number: p35: P43431 p40: P43432 Gene ID: p35: 16159 p40: 16160 Note: 1mg will be provided as 2x500μg. crossreactivity: Cow + Mouse, Human, Mouse, References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Angiogenesis/Cardiovascular Apoptosis Cancer Immune System Inflammation Neurobiology Stem Cells & Differentiation Allergy Transplantation product.subtitle.recentcitations First Author Fujita-Sato, S Title Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production. Citation The Journal of Biological Chemistry; 286(36) pg31409-17 PubMed Id 21733845 First Author Matsuda, T Title TLR9 signalling in microglia attenuates seizure-induced aberrant neurogenesis in the adult hippocampus. Citation Nature Communications; 6 pg6514 PubMed Id 25751136 First Author Chaudhry, M Z Title Cytomegalovirus inhibition of extrinsic apoptosis determines fitness and resistance to cytotoxic CD8 T cells. Citation Proceedings of the National Academy of Sciences of the United States of America; 117(23) pg12961-12968 PubMed Id 32444487 product.subtitle.productreviews Existing reviews Great product We have been using this cytokine for splenic NK cells in different publications for the past 5 years. Great consistency! From: Jin | Date: 7/21/2020 × Great product We have been using this cytokine for splenic NK cells in different publications for the past 5 years. Great consistency! From: Jin | Date: 7/21/2020 Close Great cytokine to make CD4 Th1 cells We have been using peprotech cytokines for years now. This cytokine has consistently worked for us to differentiate mouse CD4 Th1 cells. From: Chirag | Date: 8/31/2020 × Great cytokine to make CD4 Th1 cells We have been using peprotech cytokines for years now. This cytokine has consistently worked for us to differentiate mouse CD4 Th1 cells. From: Chirag | Date: 8/31/2020 Close
