Growth Factors & Cytokines

Recombinant Murine IL-12 p70

Product Details

Catalogue Number: 210-12
Description:

IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells.  It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells.  Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells.  Recombinant Murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).

Source: CHO cells

Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF

AA Sequence:

p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA

p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to induce cell proliferation in T-cell enriched PBMCs.  The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Calculated Molecular Weight: 75 kDa

Accession Number: p35: P43431 p40: P43432

Gene ID: p35: 16159 p40: 16160

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Fujita-Sato, S
Title
Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
Citation
The Journal of Biological Chemistry; 286(36) pg31409-17
PubMed Id
First Author
Matsuda, T
Title
TLR9 signalling in microglia attenuates seizure-induced aberrant neurogenesis in the adult hippocampus.
Citation
Nature Communications; 6 pg6514
PubMed Id
First Author
Chaudhry, M Z
Title
Cytomegalovirus inhibition of extrinsic apoptosis determines fitness and resistance to cytotoxic CD8 T cells.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 117(23) pg12961-12968
PubMed Id

product.subtitle.productreviews

Existing reviews
Great product
We have been using this cytokine for splenic NK cells in different publications for the past 5 years. Great consistency!
From:  Jin | Date:  7/21/2020
Great cytokine to make CD4 Th1 cells
We have been using peprotech cytokines for years now. This cytokine has consistently worked for us to differentiate mouse CD4 Th1 cells.
From:  Chirag | Date:  8/31/2020