Growth Factors & Cytokines

Recombinant Murine IL-21

Product Details

Catalogue Number: 210-21
Description:

IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include: inducing the differentiation of T cell-stimulated B cells into plasma cells and memory B cells; the stimulation of IgG production in conjunction with IL-4; and the induction of apoptotic effects in naïve B cells and stimulated B cells in the absence of T cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant Murine IL-21 is a 15.0 kDa protein consisting of 130 amino acid residues.

Source: E.coli

Synonyms: Interleukin-21, Za11

AA Sequence: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1: Measured by its ability to induce STAT3 signaling in U937 Stat3-Luc reporter cells.

Assay #2: Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Calculated Molecular Weight: 15 kDa

Accession Number: Q9ES17

Gene ID: 60505

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Finney, J
Title
Continuous Culture of Mouse Primary B Lymphocytes by Forced Expression of Bach2.
Citation
Journal of immunology (Baltimore, Md. : 1950); 207(5) pg1478-1492
PubMed Id
First Author
Szodoray, P
Title
Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity.
Citation
Cell Reports; 36(6) pg109525
PubMed Id
First Author
Sun, L
Title
Transcription factor Ascl2 promotes germinal center B cell responses by directly regulating AID transcription.
Citation
Cell Reports; 35(9) pg109188
PubMed Id

product.subtitle.productreviews

Existing reviews
Excellent product
Works very nice for mouse B cell culture
From:  Alexander | Date:  7/15/2020