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Growth Factors & Cytokines

Recombinant Murine SCF

Product Details

Catalogue Number: 250-03
Description:

SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Murine SCF is an 18.3 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Source: E.coli

Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor

AA Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.

Calculated Molecular Weight: 18.3 kDa

Accession Number: P20826

Gene ID: 17311

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

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Existing reviews
Recombinant Murine SCF
We used this when we ran out of L929-conditioned medium to produce bone marrow derived macrophages. Works equally well. Good to have as a backup.
From:  MCBL | Date:  8/7/2020
Recombinant Murine SCF
We use this product to in our media. Works well
From:  Nichole | Date:  8/26/2020