Recombinant Rat TNF-α 0 ReviewsSubmit a Review Product Details Catalogue Number: 400-14 Description: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolytically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Rat TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Source: E.coli Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin AA Sequence: MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. Calculated Molecular Weight: 17.3 kDa Accession Number: P16599 Gene ID: 24835 crossreactivity: Human, Monkey, Mouse, Mouse + Chicken, Rabbit, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Angiogenesis/Cardiovascular Apoptosis Cancer Diabetes/Weight Regulation Immune System Inflammation Neurobiology Stem Cells & Differentiation TNF Superfamily Allergy Transplantation product.subtitle.recentcitations First Author Tanabe, K Title Mechanisms of tumor necrosis factor-alpha-induced interleukin-6 synthesis in glioma cells. Citation Journal of Neuroinflammation; 7 pg16 PubMed Id 20205746 First Author van Kasteren, S I Title Glyconanoparticles allow pre-symptomatic in vivo imaging of brain disease. Citation Proceedings of the National Academy of Sciences of the United States of America; 106(1) pg18-23 PubMed Id 19106304 First Author Alirezaei, M Title Disruption of neuronal autophagy by infected microglia results in neurodegeneration. Citation PLoS ONE; 3(8) pge2906 PubMed Id 18682838