Antigen Affinity Purified Polyclonal Antibodies

Anti-Human BD-2

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Product Details

Catalogue Number: 500-P161G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBD-2. Anti-Human BD-2 specific antibody was purified by affinity chromatography employing immobilized hBD-2 matrix.

Immunogen: E.coli derived Recombinant Human BD-2 (PeproTech catalog# 300-49)

Sandwich ELISA: To detect hBD-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BD-2 (500-P161GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-2.

Anti-Human BD-2 Sandwich ELISA

Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human BD-2 Western Blot Reduced Anti-Human BD-2 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Zeeuwen, P L
Title
Genetically programmed differences in epidermal host defense between psoriasis and atopic dermatitis patients.
Citation
PLoS ONE; 3(6) pge2301
PubMed Id
First Author
Voss, E
Title
NOD2/CARD15 mediates induction of the antimicrobial peptide human beta-defensin-2.
Citation
The Journal of Biological Chemistry; 281(4) pg2005-11
PubMed Id
First Author
Bak, R O
Title
Targeting of human interleukin-12B by small hairpin RNAs in xenografted psoriatic skin.
Citation
BMC Dermatology; 11 pg5
PubMed Id