Antigen Affinity Purified Polyclonal Antibodies

Anti-Human I-309 (CCL1)

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Product Details

Catalogue Number: 500-P110
Description:
Anti-Human I-309 (CCL1)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hI-309. Anti-Human I-309 specific antibody was purified by affinity chromatography employing immobilized hI-309 matrix.

Immunogen: E.coli derived Recombinant Human I-309 (CCL1) (PeproTech catalog# 300-37)

Sandwich ELISA: To detect hI-309 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human I-309 (500-P110Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-309.

Anti-Human I-309 (CCL1) Sandwich ELISA

Western Blot: To detect human I-309 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human I-309 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human I-309 (CCL1) Western Blot Reduced Anti-Human I-309 (CCL1) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Fox, J M
Title
Structure/function relationships of CCR8 agonists and antagonists. Amino-terminal extension of CCL1 by a single amino acid generates a partial agonist.
Citation
The Journal of Biological Chemistry; 281(48) pg36652-61
PubMed Id
First Author
Pilette, C
Title
CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.
Citation
The European Respiratory Journal; 23(6) pg876-84
PubMed Id
First Author
Annunziato, F
Title
Phenotype, localization, and mechanism of suppression of CD4(+)CD25(+) human thymocytes.
Citation
The Journal of Experimental Medicine; 196(3) pg379-87
PubMed Id