Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-10

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Product Details

Catalogue Number: 500-P20

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-10. Anti-Human IL-10 specific antibody was purified by affinity chromatography employing immobilized hIL-10 matrix.

Immunogen: E.coli derived Recombinant Human IL-10 (PeproTech catalog# 200-10)

Sandwich ELISA: To detect hIL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-10 (500-P20Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10.

Anti-Human IL-10 Sandwich ELISA Anti-Human IL-10 Sandwich ELISA

Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-10 Western Blot Reduced Anti-Human IL-10 Western Blot Unreduced Anti-Human IL-10 Western Blot Reduced Anti-Human IL-10 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Aslam, A
Title
Tracking antigen-specific T-cells during clinical tolerance induction in humans.
Citation
PLoS ONE; 5(6) pge11028
PubMed Id
First Author
Bouhaddou, M
Title
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Citation
Cell; 182(3) pg685-712.e19
PubMed Id
First Author
Thivierge, M
Title
Cysteinyl-leukotriene receptor type 1 expression and function is down-regulated during monocyte-derived dendritic cell maturation with zymosan: involvement of IL-10 and prostaglandins.
Citation
Journal of immunology (Baltimore, Md. : 1950); 183(10) pg6778-87
PubMed Id