Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-4

Product Details

Catalogue Number: 500-P24

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-4. Anti-Human IL-4 specific antibody was purified by affinity chromatography employing immobilized hIL-4 matrix.

Immunogen: E.coli derived Recombinant Human IL-4 (PeproTech catalog# 200-04)

Sandwich ELISA: To detect hIL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-4 (500-P24Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-4.

Anti-Human IL-4 Sandwich ELISA

Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-4 Western Blot Reduced Anti-Human IL-4 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Blanco-Melo, D
Title
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
Citation
Cell; 181(5) pg1036-1045.e9
PubMed Id
First Author
Fong, L E
Title
Systems analysis of latent HIV reversal reveals altered stress kinase signaling and increased cell death in infected T cells.
Citation
Scientific Reports; 7(1) pg16179
PubMed Id
First Author
Fu, B
Title
Natural killer cells promote immune tolerance by regulating inflammatory TH17 cells at the human maternal-fetal interface.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 110(3) pgE231-40
PubMed Id

product.subtitle.productreviews

Existing reviews
Very stable
Works very nice in cell culture! Stable even at 4°C
From:  Steve | Date:  12/08/2021