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Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-5

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Product Details

Catalogue Number: 500-P25
Anti-Human IL-5

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-5. Anti-Human IL-5 specific antibody was purified by affinity chromatography employing immobilized Human IL-5 matrix.

Immunogen: E.coli derived Recombinant Human IL-5 (PeproTech catalog# 200-05)

Sandwich ELISA: To detect Human IL-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-5 (500-P25Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5.

Anti-Human IL-5 Sandwich ELISA

Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-5 Western Blot Reduced Anti-Human IL-5 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Chiu, W K
Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.
Journal of immunology (Baltimore, Md. : 1950); 177(11) pg7802-10
PubMed Id