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Monoclonal Antibodies

Anti-Human IL-6 (Monoclonal Mouse)

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Product Details

Catalogue Number: 500-M06
Anti-Human IL-6 (Monoclonal Mouse)


Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human IL-6. Anti-Human IL-6-specific antibody was purified from cell culture by Protein A affinity chromatography.

Source: Monoclonal Mouse

Immunogen: E.coli derived Recombinant Human IL-6 (PeproTech catalog# 200-06)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-6 (500-P26GBT) as the detection antibody at a concentration of approximately 0.10-0.20 μg/ml.

Anti-Human IL-6 Sandwich ELISA

Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions.

Anti-Human IL-6 Western Blot Reduced Anti-Human IL-6 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Di Domenico, E G
Inflammatory cytokines and biofilm production sustain Staphylococcus aureus outgrowth and persistence: a pivotal interplay in the pathogenesis of Atopic Dermatitis.
Scientific Reports; 8(1) pg9573
PubMed Id
First Author
Catelas, I
Semi-quantitative analysis of cytokines in MM THR tissues and their relationship to metal particles.
Biomaterials; 24(26) pg4785-97
PubMed Id