Monoclonal Antibodies

Anti-Human IL-7 (Monoclonal Mouse)

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Product Details

Catalogue Number: 500-M07

Source: Monoclonal Mouse

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human IL-7. Anti-Human IL-7-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography.

Immunogen: E.coli derived Recombinant Human IL-7 (PeproTech catalog# 200-07)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human IL-7 when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-7 (500-P27BT) as the detection antibody at a concentration of approximately 0.2- 0.4 μg/ml.

Anti-Human IL-7 Sandwich ELISA

Western Blot: To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-7 is 1.0-2.0 ng/lane, under reducing and non-reducing conditions.

Anti-Human IL-7 Western Blot Reduced Anti-Human IL-7 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Sakaguchi, M
Title
Overexpression of REIC/Dkk-3 in normal fibroblasts suppresses tumor growth via induction of interleukin-7.
Citation
The Journal of Biological Chemistry; 284(21) pg14236-44
PubMed Id
First Author
Cheung, K P
Title
Memory-like CD8+ T cells generated during homeostatic proliferation defer to antigen-experienced memory cells.
Citation
Journal of immunology (Baltimore, Md. : 1950); 183(5) pg3364-72
PubMed Id
First Author
Rubinstein, M P
Title
IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
Citation
Blood; 112(9) pg3704-12
PubMed Id