Antigen Affinity Purified Polyclonal Antibodies

Anti-Human MCP-1 (CCL2)

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Product Details

Catalogue Number: 500-P34
Description:
Anti-Human MCP-1 (CCL2)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF). Anti-Human MCP-1(MCAF) specific antibody was purified by affinity chromatography employing immobilized hMCP-1(MCAF) matrix.

Immunogen: E.coli derived Recombinant Human MCP-1 (CCL2) (PeproTech catalog# 300-04)

Sandwich ELISA: To detect hMCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MCP-1(MCAF) (500-P34Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF).

Anti-Human MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MCP-1 (CCL2) Western Blot Reduced Anti-Human MCP-1 (CCL2) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Pontejo, S M
Title
Two glycosaminoglycan-binding domains of the mouse cytomegalovirus-encoded chemokine MCK-2 are critical for oligomerization of the full-length protein.
Citation
The Journal of Biological Chemistry; 292(23) pg9613-9626
PubMed Id
First Author
Fitch, N
Title
Vitamin D [1,25(OH)2D3] Differentially Regulates Human Innate Cytokine Responses to Bacterial versus Viral Pattern Recognition Receptor Stimuli.
Citation
Journal of immunology (Baltimore, Md. : 1950); 196(7) pg2965-72
PubMed Id
First Author
Li, X
Title
Activation of thromboxane A2 receptor (TP) increases the expression of monocyte chemoattractant protein -1 (MCP-1)/chemokine (C-C motif) ligand 2 (CCL2) and recruits macrophages to promote invasion of lung cancer cells.
Citation
PLoS ONE; 8(1) pge54073
PubMed Id