Anti-Human MCP-1 (CCL2) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P34 Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF). Anti-Human MCP-1(MCAF) specific antibody was purified by affinity chromatography employing immobilized hMCP-1(MCAF) matrix. Immunogen: E.coli derived Recombinant Human MCP-1 (CCL2) (PeproTech catalog# 300-04) Sandwich ELISA: To detect hMCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MCP-1(MCAF) (500-P34Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF). Western Blot: To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Human, Mouse, References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Angiogenesis/Cardiovascular Cancer Chemotaxis Immune System Inflammation Neurobiology Wound Healing Allergy product.subtitle.recentcitations First Author Pontejo, S M Title Two glycosaminoglycan-binding domains of the mouse cytomegalovirus-encoded chemokine MCK-2 are critical for oligomerization of the full-length protein. Citation The Journal of Biological Chemistry; 292(23) pg9613-9626 PubMed Id 28432120 First Author Fatrai, S Title KRAS(G12V) enhances proliferation and initiates myelomonocytic differentiation in human stem/progenitor cells via intrinsic and extrinsic pathways. Citation The Journal of Biological Chemistry; 286(8) pg6061-70 PubMed Id 21169357 First Author Kim, M S Title MCP-1 is induced by receptor activator of nuclear factor-{kappa}B ligand, promotes human osteoclast fusion, and rescues granulocyte macrophage colony-stimulating factor suppression of osteoclast formation. Citation The Journal of Biological Chemistry; 280(16) pg16163-9 PubMed Id 15722361
