Antigen Affinity Purified Polyclonal Antibodies

Anti-Human MCP-2 (CCL8)

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Product Details

Catalogue Number: 500-P35

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-2. Anti-Human MCP-2 specific antibody was purified by affinity chromatography employing immobilized hMCP-2 matrix.

Immunogen: E.coli derived Recombinant Human MCP-2 (CCL8) (PeproTech catalog# 300-15)

Sandwich ELISA: To detect hMCP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MCP-2 (500-P35Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-2.

Anti-Human MCP-2 (CCL8) Sandwich ELISA

Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MCP-2 (CCL8) Western Blot Reduced Anti-Human MCP-2 (CCL8) Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Bouhaddou, M
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Cell; 182(3) pg685-712.e19
PubMed Id
First Author
Hori, T
CCL8 is a potential molecular candidate for the diagnosis of graft-versus-host disease.
Blood; 111(8) pg4403-12
PubMed Id
First Author
Fitch, N
Vitamin D [1,25(OH)2D3] Differentially Regulates Human Innate Cytokine Responses to Bacterial versus Viral Pattern Recognition Receptor Stimuli.
Journal of immunology (Baltimore, Md. : 1950); 196(7) pg2965-72
PubMed Id