Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-3. Anti-Human MMP-3-specific antibody was purified by affinity chromatography employing an immobilized MMP-3 matrix.

Immunogen: E.coli derived Recombinant Human MMP-3 (PeproTech catalog# 420-03)

Sandwich ELISA: To detect Human MMP-3 by sandwich ELISA (using 100μl/well), a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MMP-3 (500-P324BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human MMP-3.

Western Blot: To detect Human MMP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MMP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.