Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human p16-INK4a-TAT. Anti-Human p16-INK4a-TAT specific antibody was purified by affinity chromatography employing an immobilized Human p16-INK4a-TAT matrix.

Immunogen: E.coli derived Recombinant Human p16-INK4a-TAT (PeproTech catalog# 110-02T)

Sandwich ELISA: To detect Human p16-INK4a-TAT by sandwich ELISA (using100μl/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human p16-INK4a-TAT (500-P284TBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human p16-INK4a-TAT.

Western Blot: To detect Human p16-INK4a-TAT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human p16-INK4a-TAT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.