Antigen Affinity Purified Polyclonal Antibodies

Anti-Human PAI-1

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Product Details

Catalogue Number: 500-P260

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1 (human Plasminogen Activator Inhibitor-1). Anti-Human PAI-1 specific antibody was purified by affinity chromatography employing immobilized hPAI-1 matrix.

Immunogen: E.coli derived Recombinant Human PAI-1 (PeproTech catalog# 140-04)

Sandwich ELISA: To detect hPAI-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human PAI-1 (500-P260Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPAI-1.

Anti-Human PAI-1 Sandwich ELISA

Western Blot: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human PAI-1 Western Blot Reduced Anti-Human PAI-1 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Deskin, B
Title
Requirement of HDAC6 for activation of Notch1 by TGF-β1.
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Scientific Reports; 6 pg31086
PubMed Id
First Author
Andaloussi, A E
Title
Defective expression of ATG4D abrogates autophagy and promotes growth in human uterine fibroids.
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Cell Death Discovery; 3 pg17041
PubMed Id