Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human sCD14-specific antibody was purified by affinity chromatography employing an immobilized Human sCD14 matrix.

Immunogen: HEK293 cells derived Recombinant Human sCD14 (PeproTech catalog# 110-01)

Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sCD14 (500-P320Bt) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sCD14.

Western Blot: To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.