Antigen Affinity Purified Polyclonal Antibodies

Anti-Human SCF (Polyclonal Goat)

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Product Details

Catalogue Number: 500-P48G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hSCF. Anti-Human SCF specific antibody was purified by affinity chromatography employing immobilized hSCF matrix.

Immunogen: E.coli derived Recombinant Human SCF (PeproTech catalog# 300-07)

Sandwich ELISA: To detect hSCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human SCF (500-P48GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.

Anti-Human SCF Sandwich ELISA Anti-Human SCF Sandwich ELISA

Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human SCF Western Blot Reduced Anti-Human SCF Western Blot Unreduced Anti-Human SCF Western Blot Reduced Anti-Human SCF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Hsieh, F H
Title
Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 102(40) pg14380-5
PubMed Id
First Author
Dahlin, J S
Title
KIT signaling is dispensable for human mast cell progenitor development.
Citation
Blood; 130(16) pg1785-1794
PubMed Id