Antigen Affinity Purified Polyclonal Antibodies

Anti-Human sTRAIL/Apo2L

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Product Details

Catalogue Number: 500-P135

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L-specific antibody was purified by affinity chromatography employing immobilized Human sTRAIL/Apo2L matrix.

Immunogen: E.coli derived Recombinant Human sTRAIL/Apo2L (PeproTech catalog# 310-04)

Sandwich ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sTRAIL/Apo2L (500-P135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTRAIL/Apo2L.

Anti-Human sTRAIL/Apo2L Sandwich ELISA

Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human sTRAIL/Apo2L Western Blot Reduced Anti-Human sTRAIL/Apo2L Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Tatzel, K
Title
Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology.
Citation
Scientific Reports; 6 pg22661
PubMed Id
First Author
Spano, C
Title
Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer.
Citation
Scientific Reports; 9(1) pg1788
PubMed Id
First Author
Mohr, A
Title
Targeting of XIAP combined with systemic mesenchymal stem cell-mediated delivery of sTRAIL ligand inhibits metastatic growth of pancreatic carcinoma cells.
Citation
Stem cells (Dayton, Ohio); 28(11) pg2109-20
PubMed Id