Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine G-CSF

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Product Details

Catalogue Number: 500-P69

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mG-CSF. Anti-Murine G-CSF specific antibody was purified by affinity chromatography employing immobilized mG-CSF matrix.

Immunogen: E.coli derived Recombinant Murine G-CSF (PeproTech catalog# 250-05)

Sandwich ELISA: To detect mG-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine G-CSF (500-P69Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mG-CSF.

Anti-Murine G-CSF Sandwich ELISA

Western Blot: To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine G-CSF Western Blot Reduced Anti-Murine G-CSF Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Seo, S
Differential Tissue-Specific Function of Adora2b in Cardioprotection.
Journal of immunology (Baltimore, Md. : 1950); 195(4) pg1732-43
PubMed Id
First Author
Yamamoto, S
Cutting edge: Pseudomonas aeruginosa abolishes established lung transplant tolerance by stimulating B7 expression on neutrophils.
Journal of immunology (Baltimore, Md. : 1950); 189(9) pg4221-5
PubMed Id
First Author
Kreisel, D
Emergency granulopoiesis promotes neutrophil-dendritic cell encounters that prevent mouse lung allograft acceptance.
Blood; 118(23) pg6172-82
PubMed Id