Anti-Murine GM-CSF 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P65 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mGM-CSF. Anti-Murine GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix. Immunogen: E.coli derived Recombinant Murine GM-CSF (PeproTech catalog# 315-03) Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine GM-CSF (500-P65Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF. Western Blot: To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Cancer Cell Culture Inflammation Proliferation Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Kim, J Title Intratumorally Establishing Type 2 Innate Lymphoid Cells Blocks Tumor Growth. Citation Journal of immunology (Baltimore, Md. : 1950); 196(5) pg2410-23 PubMed Id 26829987 First Author Metcalf, D Title Inactivation of PU.1 in adult mice leads to the development of myeloid leukemia. Citation Proceedings of the National Academy of Sciences of the United States of America; 103(5) pg1486-91 PubMed Id 16432184 First Author Lentsch, A B Title STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia. Citation The Journal of Clinical Investigation; 108(10) pg1475-82 PubMed Id 11714739