Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-12

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 500-P155G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-12. Anti-Murine IL-12 specific antibody was purified by affinity chromatography employing immobilized mIL-12 matrix.

Immunogen: CHO cells derived Recombinant Murine IL-12 p70 (PeproTech catalog# 210-12)

Sandwich ELISA: To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-12 (500-P155GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-12.

Anti-Murine IL-12 Sandwich ELISA

Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-12 Western Blot Reduced Anti-Murine IL-12 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Maldonado Galdeano, C
Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.
BMC Gastroenterology; 11 pg64
PubMed Id
First Author
Gonzalez-Rey, E
Cortistatin, a new antiinflammatory peptide with therapeutic effect on lethal endotoxemia.
The Journal of Experimental Medicine; 203(3) pg563-71
PubMed Id
First Author
Usui, T
T-bet regulates Th1 responses through essential effects on GATA-3 function rather than on IFNG gene acetylation and transcription.
The Journal of Experimental Medicine; 203(3) pg755-66
PubMed Id